还原型谷胱甘肽对辐射损伤小鼠外周血细胞及肠损伤的影响

Effects of reduced glutathione on peripheral blood cells and intestinal injury of irradiated mice

目的观察还原型谷胱甘肽(GSH)不同给药剂量对辐射损伤小鼠外周血细胞及肠损伤的影响。方法将25只小鼠随机分为5组各5只,A1、B1、C1组照前30 min分别腹腔注射200、100、50 mg/kg GSH,D1组照前30min腹腔注射等量生理盐水,A1～D1组接受全身照射至吸收剂量为4 Gy; E1组腹腔注射等量生理盐水,不照射。照射后第15天处死小鼠并眼球取血,用血细胞分析仪计数外周血细胞,用流式细胞仪检测外周血淋巴细胞比例。另将25只小鼠随机分为A2、B2、C2、D2、E2组各5只,给药方法同上,仅照射剂量均改为10 Gy,照射方式均改为腹部局部照射。照射后第3天处死小鼠并取小肠组织,HE染色后于光学显微镜下观察小肠组织的病理学改变。结果与E1组比较,D1组外周血WBC、RBC、PLT及T淋巴细胞、B淋巴细胞比例下降(P <0. 05或<0. 01)。与D1组比较,C1组外周血RBC及T淋巴细胞、B淋巴细胞比例上升(P均<0. 05),B1组外周血WBC、RBC、PLT及T淋巴细胞、B淋巴细胞比例最高(P <0. 05或<0. 01),而A1组外周血WBC、RBC、PLT及T淋巴细胞、B淋巴细胞比例变化无统计学意义。与E2组比较,D2组小肠绒毛变短、萎缩;与D2组比较,C2组小肠绒毛较长,B2组小肠绒毛结构完整,而A2组小肠绒毛上皮仍可见脱落、绒毛排列不规则。结论 GSH能恢复受照小鼠造血功能,减轻电离辐射对小鼠小肠组织的损伤,其中GSH给药剂量100 mg/kg的辐射防护效果最佳。

Objective To observe the effects of different doses of reduced glutathione(GSH)on peripheral blood cells and intestinal injury of irradiated mice.Methods C57BL/6 mice were randomly assigned into five groups:groups A1,B1,C1,D1 and E1.The mice in the group A1,group B1,and group C1 received intraperitoneal injection of 200,100,and 50 mg/kg GSH 30 min before irradiation;mice in the group D1 were injected with normal saline intraperitoneally 30 min before irradiation;and mice in the groups A1,B1,C1,and D1 received whole body irradiation of 4 Gy;and mice in the group E1 were injected with normal saline intraperitoneally as controls.Fifteen days after irradiation,the mice were sacrificed,hematology analyzer was used to measure cell counts in the peripheral blood,and flow cytometry was used to analyze the percentage of lymphocytes in the peripheral blood.C57BL/6 mice were randomly assigned into five groups:group A2,group B2,group C2,group D2,and group E2.The method of administration was the same as before.The mice in the group A2,group B2,group C2,and group D2 received irradiation of 10 Gy for the abdominal regions.Three days after irradiation,the mice were sacrificed,the small intestines of the mice were taken out,and the pathological changes of the small intestine were observed by HE staining.Results Compared with group E1,the number of WBCs,RBCs,PLTs and the percentage of T cells and B cells in the peripheral blood of the group D1 significantly decreased(P<0.05 or P<0.01).Compared with group D1,the number of RBCs and the percentage of T cells and B cells in the peripheral blood of the group C1 increased(all P<0.05),and the number of WBCs,RBCs,PLTs and the percentage of T cells and B cells in the peripheral blood were the most highest in the group B1(P<0.05 or P<0.01),but changes in the number of WBCs,RBCs,PLTs and the percentage of T cells and B cells in the group A1 had no statistically significant difference.Compared with group E2,the small intestinal villi of the mice in the group D2 got shortened and shrunk;compared with group D2,the small intestinal villi of the mice in the group C2 were longer,and the villi structure of small intestine in the group B2 was intact,but the villi structure of small intestine in the group A2 was neither intact nor regular.ConclusionGSH can promote hematopoietic recovery in irradiated mice and reduce the the harmful effects of ionizing radiation on small intestinal mucosa,with the optimal dosage of 100 mg/kg.