DART-Q-Orbitrap MS法快速检测豆制品中碱性橙Ⅱ和金胺O

Rapid Qualitative and Quantitative Analysis of Basic Orange Ⅱ and Auramine O in Bean Products by DART-Q-Orbitrap MS

采用实时直接分析(direct analysis in real time,DART)离子化技术与四极杆-静电场轨道阱高分辨质谱联用技术(Q-Orbitrap MS),建立了快速定性和定量分析方法,检测豆制品中非法添加的碱性橙Ⅱ和金胺O。在正离子模式下,采用Full MS/targetrd-MS2扫描方式,可直接将豆制品置于离子化区域内对两种染料进行快速定性分析。在定量分析中,采用80%乙腈水溶液提取样品,应用Dip-it载样方式,对实验参数进行系统性优化。采用空白基质溶液进行梯度稀释,测得碱性橙Ⅱ和金胺O在基质中的检出限均为0.2mg/L;采用内标法定量,在1～20mg/L浓度范围内线性关系良好,相关系数分别为0.997 2和0.999 4。该方法快速准确,可为食品中非法添加染料的定性和定量检测提供参考依据。

A method of direct analysis in real time coupled with Orbitrap mass spectrometer was developed for the qualitative and quantitative analysis of Basic OrangeⅡand Auramine O in bean products.In qualitative analysis,the Full MS/Targeted MS 2 mode was applied to obtain the mass spectrum,bean products could be directly analyzed in the ionized area.DART parameters were systematically optimized to achieve the best detection performance.The component was identified by comparing with standard component and the report of relative researches as well as the full scan spectra and secondary spectra.In quantitative analysis,simple liquid extracted by 80%acetonitrile without further clean-up was used for sample preparation.Dip-it sampler was used for automatic sampling.The two pigments were subjected to gradient dilution by blank matrix solution,and both the limits of detection are 0.2 mg/L,and the linear relation in the range of 1-20 mg/L are well.The correlation coefficients are 0.997 2 and 0.999 4 for Basic OrangeⅡand Auramine O.The method is rapid and accurate,and can provide reference for the qualitative and quantitative addition of pigments in foods.