土壤霉菌菌丝球的筛选、鉴定及固定化菌丝球的应用

Screening and Identification of Soil Mycelial Pellets and Application of Immobilized Mycelium

从土壤中分离纯化真菌并鉴定为烟曲霉L-3。以菌株L-3作为固定化载体,将地衣芽孢杆菌固定在真菌上组成固定化体系。研究了混菌菌丝球,菌丝饼,发酵混合液,粗漆酶液对刚果红染料废水的降解情况并对染料废水进行毒性试验。结果表明,菌丝球对染料废水的降解效果最显著,降解率高达99.96%,菌丝饼仅用20 s降解率为91%,发酵混合液与粗漆酶液的处理效果并不显著。该体系对染料废水的去毒率较明显,尤其是菌丝球的去毒率可达到78%。可见,固定化体系对染料废水不但有较高的降解能力,也有较高的去毒率。

A fungus isolated and purified from soil was identified as Aspergillus fumigatus L-3.Using strain L-3 as immobilized carrier,on which Bacillus Licheniformis was immobilized to form an immobilized system.The degradation of Congo red dye wastewater by the composite mycelial cake,mycelia,fermentation mixture,and crude laccase enzyme solution,as well as the toxicity test of the dye wastewater were conducted.The results showed that the degradation effect on the dye wastewater by mycelial pellets was the most significant,the degradation rate reached 99.96%;and the degradation rate by mycelia cake was 91%with only 20 s,while the degradation effect by the fermentation mixture and the crude laccase solution was not so significant.The detoxification rate from dye wastewater by this system was obvious,especially the detoxification rate by mycelial pellet reached 78%.It can be seen that the immobilized system has not only high degradation ability but also a high detoxification rate.