TIM-3对食管癌细胞侵袭转移的作用机制及周期分布生物学分析

Effects of TIM-3 on Invasion and Metastasis of Esophageal Carcinoma Cells and Biological Analysis of Periodic Distribution

目的探讨TIM-3对食管癌细胞侵袭转移作用机制及周期分布生物学特性的影响。方法选取76例食管癌患者为研究对象,采用免疫组织化学法和实时荧光定量PCR法检测食管癌患者病变组织及对应的正常的癌旁组织中TIM-3水平,观察食管癌患者病变组织中TIM-3水平与淋巴结转移、肿瘤大小、肿瘤浸润深度及癌症分期的关系及其与预后的关系。结果 TIM-3 mRNA在患者正常组织和病变组织中均有表达,但在病变组织中的相对表达量明显高于正常组织,差异有统计学意义(P<0.05);患者肿瘤组织中TIM-3阳性表达率85.5%,显著高于对应的正常组织阳性表达率10.5%,差异有统计学意义(P<0.001)。癌症患者肿瘤组织中CD8^+T细胞轻微渗透30例,高度渗透46例,且TIM-3水平与CD8^+T细胞浸润深度呈负相关(γ=-0.238),差异有统计学意义(P<0.05);患者肿瘤组织中TIM-3水平与患者性别、年龄和肿瘤直径均无关(P>0.05),与临床分期、淋巴结转移和肿瘤浸润深度有关(P<0.05)。患者肿瘤组织中TIM-3高水平患者死亡率为25.0%,明显高于低水平患者的死亡率(6.6%),差异有统计学意义(P<0.05)。结论 TIM-3表达水平与食管癌的发生、肿瘤浸润深度、淋巴结转移及肿瘤分期密切相关,在一定程度上能准确反映患者肿瘤状态及预后。

Objective To provide a theoretical basis for the treatment of esophageal cancer by comparing the effects of TIM-3 on the invasion and metastasis mechanism of esophageal cancer cells and the biological characteristics of cycle distribution. Methods 69 patients with esophageal cancer were selected as subjects.TIM-3 levels in lesions and corresponding normal tissues adjacent to esophageal cancer were detected by immunohistochemistry and real-time fluorescence quantitative PCR.The lesions were observed in esophageal cancer patients.The relationship between TIM-3 level and lymph node metastasis,tumor size,depth of tumor invasion,and cancer stage and its relationship with prognosis. Results TIM-3 mRNA was expressed in both normal tissues and diseased tissues,but the relative expression level in lesions was significantly higher than that in normal tissues( P < 0.05 );3 The positive rate was 85.5%,which was significantly higher than the corresponding normal tissue expression rate of 10.5%.The difference was statistically significant( P <0.001).In cancer patients,there were 30 cases of CD8 + T cells infiltrating slightly and 46 cases of highly penetrating,and the TIM-3 level was negatively correlated with the infiltration depth of CD8 + T cells(γ=-0.238).The difference was statistically significant( P <0.05).There was no correlation between TIM-3 levels and patient gender,age,and tumor diameter( P >0.05).It was associated with clinical stage,lymph node metastasis,and depth of tumor invasion( P <0.05).The mortality rate of patients with high TIM-3 in tumor tissues was 25.0%,which was significantly higher than that of low-level patients(6.5%).The difference was statistically significant( P <0.05). Conclusion TIM-3 expression levels and esophageal cancer Occurrence,depth of tumor invasion,lymph node metastasis,and tumor stage are closely related.To a certain extent,it can accurately reflect the patient's tumor status and prognosis.