解淀粉芽孢杆菌的强启动子的克隆与鉴定

Clone and identification of a strong promoter from Bacillus amyloliquefaciens

利用启动子探针质粒,从解淀粉芽孢杆菌XH7基因组中分离得到1个强度较高的启动子P28,该启动子在大肠杆菌中表达的β-半乳糖苷酶酶活为2 350 Miller U/m L,在枯草芽孢杆菌中的酶活为3 340 Miller U/m L。启动子的序列分析表明该启动子由σK因子识别,由2个启动子串联而成,分别为编码羧基末端加工蛋白酶的基因(ctp A)和编码转醛酶的基因(tal)启动子,为芽孢杆菌启动子库提供了一个新的选择。

Using a promoter probe plasmid,promoter P28 was isolated from Bacillus amyloliquefaciens XH7,which exhibited β-Gal activity in Escherichia coli (2350 Miller U/mL) and Bacillus subtilis (3340 Miller U/mL ).Sequence analysis showed that promoter P28 contained P ctpA (a ctpA gene encoding carboxy-terminal processing protease) and P tal (a tal gene encoding translaldolase) connection in series,and it was recognized by sigma factor σK.Thus,this study provided a new strong promoter to the promoter library of Bacillus species.