哮喘患者血浆ADMA与MTP、ATP、SDH及细胞色素氧化酶活性的相关性

Association of Plasma ADMA with MTP,ATP and COX Activity in Asthmatic Patients

目的:分析哮喘急性发作患者血浆二甲基精氨酸(ADMA)水平与线粒体膜电位(MTP)、腺苷三磷酸(ATP)、琥珀酸脱氢酶(SDH)及细胞色素氧化酶(COX)活性的相关性。方法:选取哮喘急性发作患者30例为哮喘组,同期正常体检者30例为对照组,取两组被捡者清晨空腹静脉血,分离血浆和中间层(含血小板),采用高效液相法检测两组受检者的血浆ADMA水平,生物发光法测定两组的ATP含量;检测血小板MTP、ATP及SDH,分析哮喘组患者血浆ADMA水平与血小板线粒体MTP、ATP含量、SDH及COX活性的相关性。结果:哮喘组血浆ADMA水平较对照组下降,MTP、ATP、COX及SDH较对照组下降(P<0.05);哮喘组一氧化氮合酶(NOS)活性、一氧化氮(NO)含量变化与对照组比较,NO生成明显减少、NOS活性明显降低,差异有统计学意义(P<0.05);哮喘组血浆ADMA浓度与反映血小板线粒体功能的4个指标(MTP、ATP、SDH、COX)均呈负相关。结论:哮喘急性发作患者线粒体功能障碍与血浆内源性ADMA浓度升高,其机制为ADMA导致线粒体功能障碍可能与减少NO生成,增加氧化应激。

Objective:To analyze the correlation between plasma dimethylarginine level and mitochondrial transmembrane potential(MTP),adenosine triphosphoric acid(ATP),succinate dehydrogenase(SDH)cytochrome oxidase(COX)activity and in patients with acute asthma attack.Methods:30 asthmatic patients were selected as the asthma group and 30 normal subjects as the control group.The early morning fasting venous blood was collected,the plasma and the middle layer(including platelets) were separated,and the plasma ADMA levels of both groups were detected by high performance liquid chromatography and the ATP levels were measured by bioluminescence method.Platelets were detected and the correlation between ADMA level and platelet mitochondrial MTP,ATP content,SDH and COX activity was analyzed.Results:The plasma ADMA level of the asthma group was lower than that of the control group,and the changes of MTP,mitochondrial ATP,mitochondrial COX and mitochondrial SDH in the asthma group were slower than those in the control group(P<0.05).The NO production and NOS activity in the asthma group significantly decreased compared with those in the control group.(P<0.05).There was a negative correlation between plasma ADMA concentration and mitochondrial functions.ADMA/mitochondrial SDH:r=0.61,P=0.001.ADMA/cytochrome C oxidase:r=-0.69,P=0.000.ADMA/MTP:r=-0.65,P=0.000;ADMA/ATP content:r=-0.41,P=0.039.The correlation coefficients of Pearson were statistically significant(P<0.05).Conclusion:There is a correlation between Mitochondrial dysfunction is associated with elevated plasma endogenous ADMA levels in patients with acute asthma attack.The mechanism is that ADMA leads to mitochondrial dysfunction,which may be associated with a reduction in NO production and an increase in oxidative stress.