药物分子与靶蛋白相互作用的研究进展

Research advance on the interaction of pharmaceutical molecules with target proteins

药物发挥作用是以药物分子与其靶标的相互作用为基础的,对药物-靶点相互作用的定性分析与定量检测贯穿于从新药筛选发现到走向临床的整个过程。经过几十年的发展,研究药物分子与靶蛋白间相互作用的手段已经从传统的生化实验方法转变为以先进的分子生物学、生物物理学理论为支撑的高效、准确、多样化的技术体系。笔者从靶点发现与验证、亲和力测定、相互作用位点与结构分析几个方面对代表性的方法和技术进行介绍,以期为药物研发与机制探索提供参考。

The function of drugs is based on the interaction between drug molecules and their targets.Qualitative analysis and quantitative detection of drug-target interactions run through the whole process from drug discovery to clinical practice.After decades of development,the study methods on the interaction between drug molecules and target proteins have been transformed from traditional biochemical experiments to a diversity of efficient and accurate technology systems supported by advanced molecular biology and biophysics theory.In this review,representative methods and techniques were introduced from aspects of target discovery and validation,affinity determination,interaction sites and structural analysis,which might provide some references for drug discovery and mechanism exploration.