发酵麸皮多糖酶辅助提取工艺优化及其生物活性研究

Enzyme-assisted extraction of fermented wheat bran polysaccharides and it biological activity analysis

通过响应面法优化提取发酵麸皮多糖的工艺,并评价其体外益生和抗氧化活性。以发酵麸皮多糖的得率为响应值,采用纤维素酶酶解与水浴浸提相结合的方法提取发酵麸皮多糖,以纤维素酶添加量、料液比、水浴浸提温度、水浴浸提时间为试验因素建立数学模型,筛选最佳提取工艺条件。通过测定还原力、DPPH和·OH自由基的清除能力对比发酵和未发酵麸皮多糖的体外抗氧化活性,并通过测定嗜酸乳杆菌、植物乳杆菌、两歧双歧杆菌的生长对比发酵和未发酵麸皮多糖的体外益生活性。结果表明,发酵麸皮多糖最佳提取工艺为:料液比1∶16(w/v),酶添加量1 000 U/g,水浴浸提温度90℃,水浴浸提时间60 min,在此条件下发酵麸皮多糖的得率实测值为73. 35%。发酵麸皮多糖具有较强的DPPH和·OH自由基的清除能力,可促进嗜酸乳杆菌、植物乳杆菌和两歧双歧杆菌的生长。

This study was conducted to optimize enzyme-assisted extraction conditions for wheat bran polysaccharides using response surface methodology and subsequently investigated prebiotic properties and antioxidant activity of this product in vitro.A Box-Behnken design was carried out for the optimization of enzyme addition level,extraction temperature,extraction time and solid-liquid ratio.The reducing force and DPPH and·OH radical scavenging ability of fermented wheat bran polysaccharide was compared with wheat bran polysaccharide.The effects of fermented wheat bran polysaccharide on Lactobacillus acidophilus,Lactobacillus plantarum and Bifidobacterium bifidum was also compared with wheat bran polysaccharide.Optimum enzyme-assisted extraction conditions for production of wheat bran polysaccharides were:enzyme addition level,1000 U/g;extraction time,60 min;solid-liquid ratio,1∶16.The ultimate wheat bran polysaccharides yield using the optimized enzyme-assisted extraction conditions reached 73.35%.The results demonstrate that the obtained wheat bran polysaccharides could scavenge DPPH and ·OH radical and promote the proliferation of Lactobacillus acidophilus,Lactobacillus plantarum and Bifidobacterium bifidum.