苦参配方的醇提工艺优化及抑菌活性研究

Optimization of Sophorae flavescentis radix formula ethanol extraction and its bacteriostasis activity

利用响应面法设计实验优化苦参抑菌配方的回流提取工艺,以配方对金黄色葡萄球菌抑菌率作为响应值,通过考察提取工艺的单因素:浸泡时间、回流时间、水浴温度、液料比、乙醇体积分数等,选取具有显著影响的参数作为响应面实验设计变量。经过数学模型预测与验证实验确定最优提取工艺为:乙醇体积分数80. 5%、回流时间1. 6h、水浴温度95℃。在0. 003 g/m L生药给药条件下,最优工艺的醇提物的抑菌率达到94. 29%。验证结果稳定且与预测值偏差低于2%,该工艺合理可行。优化后的工艺下,所提浸膏对枯草芽孢杆菌有良好的抑制作用,对大肠杆菌抑制作用较弱。

The Response Surface was used to optimize the extraction process of Sophorae flavescentis radix antibacterial formula.The inhibition rate of Staphylococcus aureus was set uPas response value.When single factors such as soaking-time,extraction duration,reflux temperature,ratio of liquid to solid,ethanol concentration had been tested,the key factor was chosen as the variable of the Response Surface Methodology.The process of optimal extraction was determined by prediction of mathematical model and verification of experiment,which is as followed:80.5% of ethanol concentration,1.6 h of extraction duration,95 ℃ of reflux temperature.The inhibition rate of the best process is 94.29% while the concentration of raw herbal is 0.003 g/mL.The verification result is stable and the deviation from the prediction is less than 2%,which makes the extraction reasonable and feasible.Under the optimized process,the extract has a good inhibitory effect on Bacillus subtilis,and has less inhibitory effect on Escherichia coli.