摘要
目的:研究水飞蓟宾对于APP/PS1双转基因小鼠学习记忆障碍的改善作用,并从氧化应激及突触角度,探究相关机制。方法:应用Morris水迷宫实验检测水飞蓟宾对APP/PS1小鼠的空间学习记忆障碍改善作用;采用ELISA法测定脑皮质组织中MDA、GSH、SOD含量;电镜观察突触的超微结构;采用ELISAGAP-43的表达。结果:水飞蓟宾能显著改善APP/PS1模型小鼠的学习记忆障碍;降低脑内MDA含量,增加SOD、GSH含量;改善突触结构损伤;增加SYP、GAP-43蛋白的表达。结论:水飞蓟宾具有改善学习记忆障碍的作用,其作用机制可能与减轻氧化应激反应,改善突触结构和功能有关。
Objective:To investigate the effect of silibinin on learning and memory impairment in APP/PS1 transgenic mice,and to explore related mechanisms from oxidative stress and synaptic structure and function.Methods:Morris water maze test was used to detect the effect of silibinin on spatial learning and memory impairment in APP/PS1 mice.ELISA was used to detect the content of MDA,GSH and SOD in the cortex tissue.Electron microscope was used to observe the ultra-structure changes of synapses and ELISA was used to detect the expression of synapse-associated proteins SYPand GAP-43 in the cortex tissue.Result:Silibinin significantly ameliorated learning and memory impairment in APP/PS1 mice,decreased the content of MDA and increased the content of GSH and SOD in the cortex tissue,improved the ultra-structure of synapses,and increased the expression levels of synaptic related protein SYPand GAP-43 in the cortex tissue.Conclusion:The cognition-improving effects of silibinin probably result from inhibition of oxidative stress and improvement of synaptic structure and function.
作者
张礼茜
陈雪莹
范碧莲
李文博
殷浩然
顾子琨
崔岩
袁月鸣
孟禹彤
方叶
金戈
ZHANG Liqian;CHEN Xueying;FAN Bilian;LI Wenbo;YIN Haoran;GU Zikun;CUI Yan;YUAN Yueming;MENG Yutong;FANG Ye;JIN Ge(Undergraduate of Grade 2015 and 2016,Major in Clinical Medicine,Shenyang Medical College,Shenyang 110034,China;Undergraduate of Grade 2015,Major in Preventive Medicine;Department of Pharmacology)
出处
《沈阳医学院学报》
2019年第1期83-86,共4页
Journal of Shenyang Medical College
基金
辽宁省科学技术计划项目(No.20170540880)
辽宁省教育厅一般项目(No.L2014409)
沈阳市科技局(No.F13-220-9-27)
沈阳医学院大学生科研项目(No.20179011)