极低频电磁暴露对钙离子跨膜迁移的影响及机理分析

Effects of extremely low frequency electromagnetic exposure on calcium ion transmembrane migration and mechanism analysis

目的:研究极低频(ELF)电磁暴露对细胞钙离子跨膜迁移的影响。方法:以人体肝癌细胞为对象,激光扫描共聚焦显微镜为检测手段,研究细胞钙离子跨膜迁移对ELF电磁暴露的响应。结果:在磁场强度为1.78×10-7T条件下,与对照组对比,各暴露组均对不同频率和电场强度组合的外加电磁暴露环境有响应,其中f=16 Hz且EP=53 V m、f=45 Hz且EP=53 V m以及f=16 Hz且EP=80 V m的电磁暴露可使钙离子跨膜迁移量显著上升,而f=32 Hz且EP=53 V m、f=60 Hz且EP=53 V m、f=16 Hz且EP=26 V m以及f=16 Hz且EP=87 V m的电磁暴露未使钙离子跨膜迁移量显著上升。结论:不同频率和电场强度组合的ELF电磁暴露均会引起钙离子跨膜迁移量产生变化且呈现出明显差异,这可为ELF电磁环境下生物学应用提供实验依据;注重细胞膜离子通道物理、生物特性基础上探讨ELF电磁暴露对钙离子跨膜迁移影响的机理。

Objective To study the effects of extremely low frequency(ELF)electromagnetic exposure on calcium ion transmembrane migration.Methods The human hepatoma cells were taken as the subjects in this study.The responses of calcium ion transmembrane migration to ELF electromagnetic exposure were detected by laser scanning confocal microscopy.Results Under the condition that the magnetic field strength remained at 1.78×10^-7T,the transmembrane migration of calcium ion in exposure groups was responded to the applied electromagnetic exposure environment with different properties.With the frequency(f)of 16 Hz or 45 Hz and electric field peak(EP)of 53 V/m,f of 16 Hz and EP of 80 V/m,the ELF electromagnetic exposure made calcium ion transmembrane migration increased significantly,while calcium ion transmembrane migration wasn't increased significantly under the exposure with f of 32 Hz or 60 Hz and EP of 53 V/m,f of 16 Hz and EP of 26 V/m or 87 V/m.Conclusion The combination of different frequencies and electric field peaks of ELF electromagnetic exposure can increase calcium ion transmembrane migration,and the differences are significant,which provides experimental basis for the biological application of ELF electromagnetic exposure.The mechanism of the effects of ELF electromagnetic exposure on calcium ion transmembrane migration should be discussed based on the physical and biological characteristics of cell membrane ion channels.