ClC-3、BK型离子通道与胶质瘤的侵袭性生长关系

Relationship between ClC-3, BK type of ion channel and the invasive growth of glioma

目的观察ClC-3与BK离子通道在大鼠胶质瘤细胞中的表达,推测ClC-3与BK在胶质瘤细胞侵袭性生长中的作用。方法常规建立Wistar大鼠C6脑胶质瘤模型,随机分为5组(荷瘤模型组、荷瘤模型+ ClC-3阻断剂Chlorotoxin组、荷瘤模型+BK阻断剂Iberiotoxin组、假手术组及空白对照组),免疫组织化学技术检测ClC-3与BK在荷瘤动物模型中的表达情况,并进一步观察荷瘤动物在分别给予相应离子通道特异性阻滞剂后各个时间点(7、14、21 d)成瘤体积的变化情况。结果C6胶质瘤系高度表达ClC-3离子通道,而BK离子通道在该系中的表达相对较低;应用Chlorotoxin后荷瘤动物各个时间点成瘤体积与对照组相比显著降低(P<0.05),而应用Iberiotoxin后荷瘤动物的成瘤体积与对照组相比无显著变化(P>0.05)。结论 ClC-3离子通道在胶质瘤侵袭性生长发挥重要作用,可作为未来治疗胶质瘤的新靶标。

Objective To observe the expression of ClC-3 and BK ion channels in rat glioma cells, and speculate the function of ClC-3 and BK in the invasion of glioma cells. Methods Conventional establishment of C6 brain glioma model in Wistar rats, Wistar rats were randomly divided into 5 groups (glioma model group, glioma model+Chlorotoxin group, glioma model+Iberiotoxin group, sham operation group and control group). The immunohistochemical detection of ClC-3 and BK expression in glioma rat model was performed. Furthermore, the changes of tumor volume after using CIC-3 specific inhibitor Chlorotoxin and BK inhibitor Iberiotoxin were observed. Results ClC-3 ion channel was highly expressed in the C6 glioma cell line, while the BK ion channel expression was relatively low; the tumor volum of C6 brain glioma rats decreased significantly after application of Chlorotoxin (P<0.05), while there was no significant change in tumor volum of C6 brain glioma rats after the application of Iberiotoxin. Conclusion ClC-3 ion channel plays an important role in the invasive growth of glioma cells, suggesting that ClC-3 ion channels might be a new target for glioma treatment in the future.