慢性束缚应激对小鼠胃食管的影响

Effect of chronic restraint stress on gastroesophageal histological changes in mice

目的探讨心理应激在胃食管反流病(GERD)发生、发展中的作用,并为束缚应激模型的成功建立提供参考指标。方法 20只雄性SPF级昆明小鼠随机分2组(每组10只),即慢性束缚应激(CRS)组和正常对照(NC)组。CRS组小鼠每天在自制式束缚器中限制活动2 h,其余时间2组小鼠在相同环境中自由饮水摄食,实验持续14 d。实验结束后通过葡萄糖耐量试验(GTT)、胰岛素耐受试验(ITT)、小鼠胃食管组织及血清,检测相关指标确认该模型建立成功与否,同时在光学显微镜下观察胃食管黏膜组织学改变。结果 CRS组小鼠体重增加量为(8. 6±3. 1)g,明显低于NC组小鼠(12. 5±3. 0)g,差异有统计学意义(P <0. 05);胰岛素耐受试验(ITT)结果显示,CRS组小鼠出现胰岛素抵抗现象;ELISA实验显示CRS组小鼠HPA轴相关激素水平明显高于NC组,差异有统计学意义(P <0. 05)。CRS组小鼠胃食管黏膜可见充血、水肿及炎症细胞的浸润。结论 CRS可影响机体,引起GERD相关组织病理学变化。为阐述心理因素在GERD中的作用机制研究提供简单有效并且具有借鉴意义的实验基础。

ObjectiveTo discuss the effect of chronic restraint stress(CRS)on gastroesophageal histological changes in mice for investigating the role of psychological factor in the pathogenesis of gastroesophageal reflux disease,and provide with referential parameters for successful establishment of CRS model.Methods 20male KunMing mice were randomly divided into two groups which are CRS and normal control(NC)group.Mice in CRS group were submitted to partial restraint stress for 2 h per day.After 14 days of restraint stress,glucose tolerance test(GTT)and insulin tolerance test(ITT)were performed firstly,and esophagus,stomach and blood sample of all mice were taken for further detecting the parameters and analyzing histological changes in tissues through HE staining.ResultsBody weight increment of mice in CRS group is significantly lower than that of NC group(P<0.05).ITT shows that CRS group happened insulin resistance.The result of ELISA indicated that the level of hormones related to HPA axis in CRS group was significantly higher than those of NC mice(P<0.05).Histological changes appeared in CRS group include hyperemia,edema and infiltration of inflammatory cells in gastroesophageal mucosa.ConclusionCRS obviously affects physiological activities and may result in GERD-related histopathological changes in stomach and esophagus.Our study provides with not only simple,but also effective experimental basis which has referential value on the research of investigating the role of psychological factor in the pathogenesis of GERD.