光交联羧甲基壳聚糖衍生物水凝胶的制备及性能研究

Preparation and Characterization of Photo-Crosslinking Hydrogel of Carboxymethyl Chitosan Derivative

以叠氮基团的光耦合反应为交联反应制备了叠氮化羧甲基壳聚糖(AZ-CMCS)水凝胶。利用FT-IR红外光谱、1H-NMR核磁共振谱表征了AZ-CMCS的结构,使用紫外可见分光光度计和流变仪跟踪了AZ-CMCS凝胶的光交联过程,并验证了凝胶的可注射性.使用水接触角测试仪表征了凝胶的亲疏水性.以牛血清白蛋白(BSA)为模型药物研究了水凝胶在不同pH值下的药物释放性能,并研究了人胚胎肺成纤维细胞(MRC-5)在水凝胶上的生长情况.结果表明:通过紫外光照射可快速得到AZ-CMCS水凝胶,反应条件温和,易于控制;AZ-CMCS水凝胶具有较好的可注射性,且具有一定的pH响应性,其能够实现包载药物和药物控释的功能;此外AZ-CMCS水凝胶对细胞无毒,可作为细胞支架,具有较好的应用前景.

The azide carboxymethyl chitosan(AZ-CMCS)hydrogel was prepared by UV-irradiation-induced photo-crosslinking reaction which was based on the optical coupling reaction of azide groups.The chemical structure of the AZ-CMCS hydrogel was characterized by FT-IR and 1 H-NMR.The photo-crosslinking process was tracked by a rheometer and a UV-Vis spectrophotometer.The injectable property of the AZ-CMCS hydrogel was verified by a rheometer.The hydrophobicity of the AZ-CMCS hydrogel was assessed by a water contact tester.Bovine serum albu-min was used as the model drug to analyze the drug release property of the AZ-CMCS hydrogel at different pH values.The growth condition of human embryonic lung fibroblast(MRC-5)cells on AZ-CMCS scaffolds was also investigated.Results showed that the AZ-CMCS hydrogel can be rapidly prepared by UV irradiation.The reaction conditions are mild and controllable.The AZ-CMCS hydrogel has good injectable properties and pH sensibility.The AZ-CMCS hydrogel can load the model drug and control the drug release behavior.Moreover,the AZ-CMCS hydrogel is non-cytotoxic and has a good application prospect as cell scaffold.