泾阳茯茶生产环境中冠突散囊菌多样性检测

Identification of Eurotium amstelodam Isolated from Jingyang Fu Tea and Its Processing Environment

为阐明陕西省泾阳县茯茶生产环境中冠突散囊菌是否为同一克隆系,分别从泾阳县2个代表不同生产方式的茯茶厂的不同生产工段采集原料茶叶、茯茶半成品及成品、生产环境涂抹拭子、空气沉降样和土壤等样品,采用平板划线和涂布法分离得到26株疑似冠突散囊菌,并对其进行分类鉴定。结果表明:基于菌落形态特征鉴定后,初步确定26株菌为"冠突散囊菌"。对26株"冠突散囊菌"26SrDNA D1/D2区及其内转录间隔区(Internal Transcribed Spacer;ITS)序列进行PCR扩增、序列测定并提交基因库比对,使用Mega 6.0软件构建进化树分析鉴定后,确认得到的菌株均为冠突散囊菌,且菌株的26SrDNA D1/D2区及ITS序列均具有高度的同源性。研究初步阐明存在于陕西省泾阳县茯茶生产环境的冠突散囊菌相似度极高,为同一克隆系。

Twenty six presumptive Eurotium amstelodam isolates that recovered from raw tea,semi-processed Fu tea,Fu tea products,swabs of the environment,air in two Fu tea processing factories,and soil of the surroundings of the factories by streak and coating method,in Jiangyang,Shaanxi province,were identified in this study to reveal the diversity of Eurotium amstelodam in this district.The 26 strains were preliminarily identified as Eurotium amstelodam via observing colony and mycelia morphological characteristics by microscope.After the sequences of D1/D2 region and the internal transcribed spacer(ITS)of 26S rDNA in 26 presumptive Eurotium amstelodam strains were amplified,sequenced and compared with those submitted in NCBI database,phylogenetic tree was made and confirmed these 26 strains were all Eurotium amstelodam.Our results exhibited that Eurotium amstelodam existed in various environments of Fu tea processing factories in Jingyang,Shaanxi,and the isolates in these environment settings were highly similar,which supported the possibility for Fuzhuan tea industrial producing by Eurotium amstelodam inoculation.