MTT比色法测定烟酰胺类化合物对植物病原细菌的抑菌活性

Determination of the Nicotinamide Compounds by Using MTT Colorimetric Assay against Pathogenic Bacteria

【目的】探究MTT比色法用于合成化合物对植物病原细菌抑菌活性筛选的可行性。【方法】将7个烟酰胺类化合物分别用MTT比色法和平板扩散法对番茄疮痂病菌(Xanthomonas vesicatoria)、烟草青枯病菌(Ralstonia solanacearum)和丁香假单胞杆菌(Pseudomonas syringae)的抑菌活性进行筛选并测定其有效中浓度EC50。【结果】采用平板扩散法进行抑菌活性初筛时,未能筛选出有活性的化合物;采用MTT比色法筛选出N-(3-三氟甲基)-苯基烟酰胺具有抑菌活性,其对番茄疮痂病菌(X. vesicatoria)、烟草青枯病菌(R. solanacearum)和丁香假单胞杆菌(P. syringae)的EC50分别为3.87、2.05、10.33 mg/mL。【结论】MTT比色法能更加准确地评价脂溶性化合物对细菌的抑菌活性,并具有广泛的应用前景。

[Purpose]To investigate the possibility of antibacterial activity of 7 synthetic nicotinamide compounds against 3 kinds of pathogenic bacteria by MTT assay.[Methods]We evaluated bactericidal activity of 7 synthetic nicotinamide compounds against Xanthomonas vesicatoria,Ralstonia solanacearum and Pseudomonas syringae by MTT assay and plate diffusion method.MTT assay performed to identify bactericidal activities of N-(3-(trifluoromethyl)phenyl)nicotinamide.[Result]The EC50 of N-(3-(trifluoromethyl)pheny)nicotinamide against X.vesicatoria,R.solanacearum and P.syringae by MTT assay were 3.87,2.05 and 10.33 mg/mL.[Conclusion]The results indicated that MTT assay was more effective than plate diffusion method in evaluating bactericidal activity,and has higher value on application.