数字PCR技术及其在微生物检测中的应用

Research Progress on the Application of Digital in Microbial Detection

数字PCR是继实时定量PCR之后新兴的一种绝对定量分析技术。近年来,世界各国都非常重视数字PCR检测技术的研究和应用。因此,有必要建立快速简单、高灵敏度、高精确度的微生物检测方法。本文介绍了数字PCR技术的基本原理,概述了其在微生物检测领域的应用进展。

The digital PCR(dPCR)is a new developed absolute quantitative analysis technology after real-time PCR.In recent years,all countries in the world have attached great importance to the research and application of dPCR detection technology.Therefore,it is very necessary to establish a fast,simple,highly sensitive and accurate microbiological detection method.In this paper,the basic principle of dPCR technology is introduced,and its application in the field of microbial detection is summarized.