摘要
数字PCR是继实时定量PCR之后新兴的一种绝对定量分析技术。近年来,世界各国都非常重视数字PCR检测技术的研究和应用。因此,有必要建立快速简单、高灵敏度、高精确度的微生物检测方法。本文介绍了数字PCR技术的基本原理,概述了其在微生物检测领域的应用进展。
The digital PCR(dPCR)is a new developed absolute quantitative analysis technology after real-time PCR.In recent years,all countries in the world have attached great importance to the research and application of dPCR detection technology.Therefore,it is very necessary to establish a fast,simple,highly sensitive and accurate microbiological detection method.In this paper,the basic principle of dPCR technology is introduced,and its application in the field of microbial detection is summarized.
作者
吴冰
蔡先全
郑传发
邱霞
萧绮倩
Wu Bing;Cai Xianquan;Zheng Chuanfa;Qiu Xia;Xiao Qiqian(Zhongshan Technical Center of Zhongshan Entry-Exit Inspection and Quarantine Bureau,Zhongshan 528403,China)
出处
《现代食品》
2018年第22期77-79,共3页
Modern Food