蓝莓果渣中花色苷的超临界二氧化碳萃取工艺优化

Optimization of extracting technology of anthocyanins from blueberry pomace by supercritical carbon dioxide

采用超临界二氧化碳萃取技术提取蓝莓果渣中的花色苷,通过4因素3水平的Box-Behnken中心组合实验优化提取工艺。结果显示,各因素对蓝莓果渣中花色苷的影响顺序为:萃取时间>萃取压力> CO2流率>萃取温度,当萃取温度40℃,萃取时间1. 86 h,萃取压力34. 7 MPa,CO2流率4. 5 L/min时,蓝莓果渣中花色苷的得率最高达1. 483 6 mg/g,与模型预测的最大得率误差仅为0. 095%,验证了模型的可靠性。超临界二氧化碳萃取的花色苷纯度较高,用体积分数60%的乙醇溶液溶解时,色价达E1%1 cm(530. 8 nm)达41. 58。

The supercritical dioxide extraction was used to extract anthocyanins.The extraction technology was optimized by the Box-Behnken center combinations response surface test based on the single factors experiments.The results showed that the order of the influence factors on the anthocyanins extraction as follows:extraction time>extraction pressure>CO 2 flow rate>extraction temperature.When the extraction temperature was 40℃,the extraction time 1.86 h,the extraction pressure 34.7 MPa,and the CO2 flow rate 4.5 L/min,the yield of anthocyanins in blueberry residual was the highest(1.483 6 mg/g),which was only 0.095%error of the prediction model,and verified the reliability of the model.The purity of anthocyanins extracted by supercritical carbon dioxide was better,and the color characteristic of E 1%1 cm(530.8 nm)reached to 41.58.