血小板对人肺癌细胞株A549凋亡的影响及机制初探

The Influence of Platelets on Apoptosis of Lung Cancer Cells

目的探讨血小板(PLT)对肺癌细胞株A549的影响及其可能的机制。方法通过流式细胞术检测PLT对A549细胞的凋亡作用;Westernblot检测PLT和A549细胞共培养后A549细胞中ERK1/2、JNK、p-ERK、p-JNK、MMP–2、M M P–9、 B c l–2、 B a x蛋白的表达情况。结果应用流式细胞术的检测结果发现,血小板抑制A 549细胞的凋亡并能阻止化疗药物吉西他滨诱导的A549细胞凋亡。Westernblot结果显示PLT和A549细胞共培养48h后,A549细胞中ERK1/2、JNK、p-E R K、 p-J N K、 M M P-2、 M M P-9、 B c l-2蛋白表达明显增加, B a x蛋白表达明显降低。结论血小板可增加A 549细胞中ERK1/2、JNK、p-ERK、p-JNK磷酸化,增加MMP-2、MMP-9和Bcl-2蛋白的表达;降低Bax蛋白表达,并抑制肺癌细胞株A549的凋亡。血小板可抑制A549细胞的凋亡,且与血小板的浓度呈正相关。

Objective To study the impact of platelet on tumor cell apoptosis which is associated with resistance of lung cancer cells to chemotherapy.Methods Flow cytometry was performed to detect the apoptosis of A549 induced by PLT.The expression of ERK1/2,JNK,p-ERK,p-JNK,MMP 2,MMP-9,the Bcl-2,Bax was examined by Western blotting after co-culture of PLT with A549 cells.Results The expression of ERK1/2,JNK,p-ERK,p-JNK,MMP-2,MMP-9,and Bcl-2 was significantly upregulated while Bax was decreased after 48h cultrue.Conclusions Platelets facilitated migration of A549 cells in vitro,which was positively correlated with the platelet concentrations.Platelets increased the phosphorylation of ERK1/2,JNK,p-ERK,p-JNK;MMP-2 and the expression of MMP-2,MMP-9,and Bcl-2,but decreased the expression of Bax protein and inhibited the apoptosis of lung adenocarcinoma cells.