不同方式放置白膜制备浓缩血小板质量指标比较

Study on the Qualities of Two Kinds Buffy-Coat-Collected Platelet Concentrates

目的探讨全血分离白膜静置2h后,血小板恒温保存箱振荡过夜保存白膜袋及静置室温过夜保存白膜袋,对制备浓缩血小板回收率及血小板质量指标的影响。方法对124份400mL新鲜全血进行容量检测和血常规计数,随机选取其中53份当日分离白膜静置2h后置血小板恒温保存箱振荡过夜保存为实验组,剩余71份当日分离白膜后静置室温过夜保存为对照组,两组白膜于次日制备浓缩血小板。然后对两组浓缩血小板进行p H、葡萄糖、血小板聚集率及血小板低渗休克反应相对变化率(HSR)的测定。结果白膜振荡过夜保存和静止过夜保存后制备的浓缩血小板计数分别为(7.23±2.21)×10^(10)和(7.17±2.08)×10^(10),实验组高于对照组,差异无统计学意义。但是血小板回收率则是实验组为68.69%±19.21%,高于对照组血小板回收率62.07%±11.37%,差异有统计学意义。实验组和对照组制备的浓缩血小板pH值分别为7.23±0.08和7.12±0.14,GLU(mmol/L)分别为20.57±1.26和19.32±1.41,血小板聚集率分别为92.65%±3.02%和88.82%±3.43%,差异均无统计学意义。实验组浓缩血小板HSR为81.50%±8.57%,明显高于对照组浓缩血小板74.30%±11.55%,差异有统计学意义。结论两种方式放置白膜袋所制备的浓缩血小板,检测指标均符合国家质量标准,但血小板回收率和体外功能检测指标HSR显示白膜静置后振荡过夜方式制备的浓缩血小板可能更具有优势。

Objective This study was aimed to compare the difference of qualities between two kinds Buffy-Coat-Collected Platelet Concentrates.Methods 400ml of whole blood were harvested from each of 124 healthy donors and then were separated and prepared to obtain the platelets through buffy coat method.124 buffy coats were divided into 2 groups randomly,53 buffy coats in experiment group were agitated overnight after standing 2 hours in platelet agitating container,while 71 buffy coats in control group were stewing overnight at room temperature.pH,GLU,HSR,platelet aggregation rate were observed in all platelet concentrates.Results There was no difference in platelet counts,pH and platelet aggregation rate between the two groups(P>0.05).However,compared with the control group,platelets collected from agitated buffy coats had higher ability to hypotonic shock response and a higher rate of platelet recovery(P<0.05).Conclusion Two kinds of buffy-coat-collected platelet concentrates meet the requirement of relevant standards.Platelet concentrates from agitated buffy coats maybe have more advantages over those from stewing buffy coats in HSR and platelet recovery rate.