一株采油用嗜热特基拉芽孢杆菌厌氧生长代谢研究

Study on Anaerobic Growth Metabolism of a Bacillus Tequilensis Strain Used for Oil Recovery

为了提高外源微生物油藏适应性和采油能力,以一株采油用嗜热特基拉芽孢杆菌(Bacillus tequilensis)为对象,对其厌氧生长代谢和驱油功能进行研究。该菌株为兼性微生物,在好氧和厌氧条件下都能生长代谢,在好氧条件下生长较快,12 h到达峰值期,而厌氧条件下需要124 h,通过厌氧连续传代驯化后菌株厌氧生长代谢速率提高2. 7倍;厌氧条件下提供电子受体可以提高菌株的乳化和产气能力,该菌株厌氧乳化指数达到100%,乳化剂产量为0. 75 g/L,所产乳化剂为大分子多糖蛋白类复合物;该菌株厌氧代谢过程中同时产生二氧化碳气体和挥发性脂肪酸,随着电子受体量的增加,产气量增加而脂肪酸产物浓度降低。该菌株物模评价水驱基础上提高采收率达到16. 1%,展现出良好的采油应用潜力。

In order to improve the adaptability and oil recovery ability of exogenous microorganism,the anaerobic growth and metabolism and oil displacement function of a strain of thermophilic Bacillus tequilensis was studied.This strain is facultative bacteria,which could grow and metabolize under both aerobic and anaerobic conditions.It grows rapider under aerobic conditions,its growth peak reaches at 12 hours,while under anaerobic conditions the growth peak reaches at 120 hours.The anaerobic growth and metabolism rate of the strain was increased by 2.7 times after continuous generation.Electron acceptor could improve the emulsification and gas production ability of the strain under anaerobic conditions.The anaerobic emulsification index of the strain reached 100%,the yield of emulsifier was 0.75 g/L,and the emulsifier was macromolecule polysaccharide protein complex.The strain produced both carbon dioxide and volatile fatty acids during anaerobic metabolism,the carbon dioxide production increased and the concentration of the fatty acid products decreased with the increase of electron acceptor.The physical simulation experiment shows that the strain can enhance oil recovery by 16.1% on the basis of water flooding,showing good oil recovery potential.