珠海口岸地区孕妇人群梅毒螺旋体基因分型的初步研究

Preliminary study on Treponema pallidum genotyping of pregnant women in Zhuhai port area

目的分析珠海口岸地区孕妇人群的梅毒螺旋体感染情况及基因分型,探讨先天性梅毒与梅毒基因分型的关系。方法统计2016―2017年该院建档孕妇的梅毒检测例数为4 414例,其中42例被确诊为现状梅毒,其新生儿有9例患有先天性梅毒。对梅毒阳性患者血液进行巢式PCR检测arp、tpr基因并进行分型。结果 42例现状梅毒孕妇有25例检测出polA基因阳性,9例先天性梅毒患儿检测出7例polA基因阳性;最后综合基因分型14a型2例(6%,2/32),13d型5例(16%,5/32),14d型25例(78%,25/32),其中7例polA基因阳性的先天性患儿和母亲的型别全为14d型。结论珠海口岸地区梅毒流行基因型主要为14d型,同样在先天性梅毒中基因型也是14d型。

Objective To analyze the status and genotyping of Treponema pallidum infection in pregnant women in Zhuhai port area,and to investigate the relationship between congenital syphilis and syphilis genotyping.Methods The number of syphilis test cases of pregnant women in our hospital was 4 414 from 2016 to 2017,of which 42 cases were diagnosed with current syphilis,their newborn babies,and 9 cases with congenital syphilis.Nest PCR was used to detect the meltox arp gene and tpr gene in the blood of syphilis positive patients and to classify them.Results A total of 25 cases of pregnant women with syphilis positive for polA gene,and 9 cases of congenital syphilis tested positive for polA gene in 7 cases.The final comprehensive gene classification type 14a had two cases(6%,2/32),13d type had 5 cases(16%,5/32),14d type had 25 cases(78%,25/32).Among them,seven cases of congenital children and mothers with polA gene positive were all type 14d.Conclusion Epidemic genotype of Zhuhai port area syphilis is mainly 14d,as well as in congenital syphilis genotype.