摘要
目的:探讨3种微柱凝胶抗人球蛋白试剂卡的检测效力,评价其灵敏度差异,为临床试验选择合适的试剂卡提供理论依据。方法:随机选取本院交叉配血次侧不合住院病人标本,运用直接抗人球蛋白试验方法对3种试剂卡进行平行检测。结果:3种试剂卡检出率比较存在显著性差异(P <0. 05),A、B卡与C卡比较均存在显著性差异(P <0. 05)但A卡与B卡比较无显著性差异(P> 0. 05)。对于A、B卡检测为弱阳性的标本,用C卡检测普遍为阴性。对于弱阳性标本,凝集强度A卡> B卡> C卡;对于强阳性标本,凝集强度B卡> A卡> C卡。检测灵敏度B卡> A卡> C卡。结论:推荐临床各实验室使用B卡对患者标本进行输血相容性检测,辅以A卡与C卡进行比较和排异。
Objective:To investigate the detection efficacy of three kinds of microcolumn gel antihuman globulin test kits,and to evaluate their sensitivity differences,so as to provide a theoretical basis for selecting suitable test kits in clinical trials.Methods:Samples of inpatients with incompatible secondary side of cross matching were randomly selected,and three test kits received parallel detection by direct anti-human globulin test.Results:There were significant differences in the detection rate of three test kits(P<0.05),and there were significant differences between A,B kits and C kits(P<0.05),but there was no significant difference between A and B kits(P>0.05).For weakly positive specimens detected by A and B kits,C kit was generally negative,for weakly positive specimens,agglutination strength ranking was A kit>B kit>C kit,for strongly positive specimens,agglutination strength ranking was B kit>A kit>C kit.Detection sensitivity ranking was B kit>A kit>C kit.Conclusion:It is recommended that clinical laboratories use B kit to test the blood transfusion compatibility of patients.specimens,which is supplemented by A kit and C kit for the comparison and exclusion.
作者
杨光
刘自强
吴艳红
杨静
王岚
Yang Guang;Liu Ziqiang;Wu Yanhong;Yang Jing;Wang Lan(Department of Blood Transfusion,Sichuan Provincial People s Hospital,Chengdu Sichuan,610031,China)
出处
《现代临床医学》
2019年第1期42-43,61,共3页
Journal of Modern Clinical Medicine
关键词
微柱凝胶抗人球蛋白试剂卡
直接抗人球蛋白试验
交叉配血
次侧不合
检测灵敏度
microcolumn gel antihuman globulin test kit
direct antihuman globulin test
cross matching
incompatibility of secondary side
detection sensitivity