香菇0912液体菌种培养基及摇瓶发酵条件的筛选

Screening of Liquid Medium and Fermentation Conditions for Lentinus edodes 0912 by Shaking Flask

不同菌种对液体培养基中营养物质的利用效率不同,试验以香菇0912作为供试菌株,以菌丝体干质量为指标,通过单因素试验,研究了不同碳氮源、无机盐含量、转速、培养基初始pH、静置时间以及培养基装液量对香菇菌丝干质量的影响,结果表明最佳碳源为葡萄糖,最佳氮源为玉米浆,K_2HPO_4的最佳用量为0.1%,KH_2PO_4的最佳用量为0.1%,初始pH值5.0,装液量为50 mL,培养温度为25℃,静置时间为24 h,转速为180 r·min-1。在此基础上,对液体培养基的组分含量进行了L9(34)正交试验,结果表明4个因素对香菇菌丝干质量的影响表现为:葡萄糖>玉米浆>KH_2PO_4>K_2HPO_4,其中葡萄糖和玉米浆的含量对菌丝干质量具有极显著影响(P<0.01),并确定了香菇0912菌株液体深层发酵各组分的优水平为葡萄糖3%,玉米浆0.3%,K_2HPO_40.05%,KH_2PO_40.05%,搭配MgSO40.05%,维生素B10.005%,在此条件下,菌丝干质量达到2.103 g·L-1,是优化前(1.417 g·L-1)的1.49倍。

The utilization efficiency of nutrients in liquid medium was different among different strains.In the study,Lentinus edodes 0912 was used as the tested strain.The optimal carbon and nitrogen sources,inorganic salt content,rotational speed and initial pH of culture medium,and also the standing time and medium volume were screened through the single factor experiment,and the dry weight of mycelia was as the evaluation index.The results showed that the best carbon source was glucose,the best nitrogen source was corn pulp,the best amount of K2HPO4 and KH2PO4 was both 0.1%,the initial pH was 5.0,the liquid medium volume was 50 mL,the culture temperature was 25℃,the rest time was 24 h and the speed was 180 r·min^-1.On the basis,the composition of liquid medium was tested by L9(3^4)orthogonal test.The results showed that the effects of four factors on the dry weight of L.edodes were glucose>corn pulp>KH2PO4>K2HPO4,in which the content of glucose and corn pulp had a significant effect on the dry weight of mycelium(P<0.01),and the optimum conditions for liquid deep fermentation of L.edodes strain 0912 were glucose 3%,corn pulp 0.3%,K2HPO4 0.05%,KH2PO4 0.05%,matched with MgSO4 0.05%and VB1 0.005%,the dry weight of mycelium could reach 2.103 g·L^-1,which was 1.49 times of that before optimization(1.417 g·L^-1).