连续离子交换色谱分离甘草酸和甘草黄酮

Separation of glycyrrhizic acid and licorice flavonoids by continuous ion exchange chromatography

以甘草酸和甘草黄酮的吸附率及解析率为指标,探究了6种树脂对二者吸附和解析性能,筛选了最佳分离树脂。考察了影响二者分离效果的条件,确定了连续离子交换色谱分离二者的最佳工艺。结果表明,D941是分离甘草酸和甘草黄酮的最佳树脂。最佳分离工艺为:上样质量浓度1.0mg/mL,体积2.70L,体积流量12.5mL/min;乙醇洗脱体积分数70%,体积1.80L,体积流量12.50mL/min;NaOH洗脱浓度0.50mol/L,体积0.6L,体积流量5.0mL/min。在最佳工艺条件下制得甘草酸纯度为76.53%,得率2.09%;甘草黄酮纯度为67.33%,得率2.68%。结果说明连续离子交换色谱可以实现甘草酸与甘草黄酮的同时分离,提高生产效率。

With the adsorption and desorption rates of glycyrrhizic acid (GA) and licorice flavonoids (LF) as indicators,the adsorption and desorption properties of six resins were investigated,and the optimum separation resin was selected.The parameters affecting the separation efficiency were investigated,and the optimum process for separation of the two compounds by continuous ion exchange chromatography (CIEC) were determined.The result showed that D941 was the best resin for separating GA and LF.The optimum separation processes of continuous ion exchange chromatography are as follows: the mass concentration of sample of 1.0 mg/mL,volume of 2.70 L,volume flow of 12.5 mL/min;the volume fraction of ethanol elution of 70%,volume of 1.80 L,volume flow of 12.50 mL/min;NaOH elution concentration of 0.50 mol/L,volume of 0.6 L,volume flow of 5.0 mL/min.Under the optimum conditions,GA was obtained with purity of 76.53% and yield of 2.09%,while LF was obtained with purity of 67.33% and yield of 2.68%.The results indicated that CIEC can separate GA and LF simultaneously and improve production efficiency.