基于TaqMan PCR鉴定猪源性成分的引物和探针研发

Research and development of primer and probe for identification of porcine-derived materials inferred from TaqMan PCR

为了研发具有自主知识产权的猪源性成分检测的引物和探针,比对猪、牛、羊、马、鸡、鸭、兔、鹅等8种动物的线粒体基因组,筛选并设计猪特异性的引物和探针组合进行荧光定量PCR。研究结果表明,所研发的猪源性成分检测的引物和探针仅对猪相关DNA模板有典型扩增曲线,对其他动物来源的DNA模板无扩增,具有高度的猪源性特异性。灵敏度实验表明,利用此引物和探针的检测方法具有高度的灵敏度,可达1pg级。肉制品中猪源性定量检测实验说明此方法具有较好的定量检测能力。此引物和探针组合适用于猪源性食品和农畜产品的检验检测。

The objective of this study was to design the primer and probe with intellectual property which can be used to detect porcine-derived materials by means of Taq Man PCR.The results showed that the technique based on the primers and probe was specific for species identification and quantification,and was able to detect 1 pg template.This biotechnological method based on Taq Man PCR was appropriate to identify porcine-derived materials in farm products and foods.