小麦TaeEF1β基因的克隆、同源性及表达分析

Gene cloning,homology and expression analysis of TaeEF1β in Triticum aestivum L.

真核翻译延伸因子(eukaryotic translation elongation factor,e EFs)是一种重要的多功能调控蛋白,eEF1β是eEF1的组成部分,在蛋白质生物合成过程中发挥着重要的作用。本文通过RT-PCR扩增克隆小麦(Triticum aestivum L.)的e EF1β基因,并命名为TaeEF1β。氨基酸同源性分析发现,TaeEF1β具有高度保守性,且其保守结构域位于137～226 aa处。q RT-PCR结果表明,中国小麦花叶病毒(Chinese wheat mosaic virus,CWMV)侵染小麦植株后,可以诱导TaeEF1β基因转录水平的上调表达。另外,本文也进一步分析了TaeEF1β基因在小麦根、茎、叶的表达水平和CWMV侵染不同时间点的表达情况。

Eukaryotic translation elongation factor(eEFs)is an important multifunctional protein.eEF1βwas subunit of the eukaryotic translation elongation factor-1(eEFs-1)complex and played an important role in protein biosynthesis.The eEF1βgene was obtained by cloning with RT-PCR from wheat and named as TaeEF1β.The amino acid sequences of Ta eEF1βwere highly conserved and the conserved domain was located in 137-226 aa.The results of qRT-PCR showed that TaeEF1βwere up-regulated in the CWMV-infected plants.In this study,the expression of TaeEF1βin the stems,leaves and roots of wheat and different stages of CWMV infection was also detected by qRT-PCR.