菜粉蝶气味结合蛋白和化学感受蛋白生物信息学分析

Bioinformatics analysis of odorant-binding proteins and chemosensory proteins of Pieris rapae

气味结合蛋白(odorant-binding proteins,OBPs)和化学感受蛋白(chemosensory proteins,CSPs)在菜粉蝶(Pieris rapae)气味识别的过程中发挥着重要作用。通过本地BLAST等方法在菜粉蝶基因组中找到了推测的OBPs和CSPs蛋白序列,采用多序列比对、半胱氨酸模式序列识别、进化发育树构建等方法,鉴定出了24个PrOBPs和38个PrCSPs基因。其中,PrOBP1-PrOBP17这17个OBPs属于Classic OBPs蛋白家族,PrOBP19-PrOBP24属于Minus-COBPs蛋白家族。鉴定出的38个PrCSPs都具有鳞翅目CSPs的半胱氨酸模式识别序列。本实验通过对菜粉蝶基因组中OBPs和CSPs基因的查找鉴定,丰富了昆虫OBPs和CSPs基因数据库,可为菜粉蝶气味识别和化学感受相关实验和应用提供参考。

Odorant-binding proteins(OBPs)and chemosensory proteins(CSPs)play an important role in the recognition of odors.The putative OBPs and CSPs protein sequences were found in the cabbage butterfly genome by the local BLAST method.We identified 24 PrOBP s and 38 PrCSP s genes using multiple sequence alignments,cysteine pattern sequence recognition,and evolutionary tree construction.Among them,the 17 OBPs of PrOBP1-PrOBP17 belonged to the Classic OBPs protein family,and the other six OBPs,PrOBP19-PrOBP24,belonged to the Minus-C OBPs protein family.All 38 PrCSPs identified had cysteine pattern recognition sequences of typical lepidoptera CSPs.In this paper,the search and identification of OBP s and CSP s genes in the cabbage butterfly genome enriched the database of insect OBP s and CSP s genes,and also provided references for odor identification and chemical sensing experiments and applications of Pieris rapae.