紫外-可见光分光光度法测定八月瓜果皮齐墩果酸

Content Measurement of Oleanolic Acid in Akebia Trifoliata Pericarp by Ultraviolet-Visible Spectrophotometry

为了建立齐墩果酸的紫外光谱分析方法,以香草醛-冰醋酸为显色剂,545nm为测定波长,用标准比较法考察了齐墩果酸含量的紫外光谱分析条件.结果表明,用0.3 mL 5%香草醛-冰醋酸做显色剂,70℃下恒温显色15 min,8.00mg/L齐墩果酸标准溶液的实验测定值为8.02mg/L;齐墩果酸在2.00～16.00mg/L范围内线性关系良好,相关系数为0.999 8.以质量比计,用本方法测定的八月瓜果皮中齐墩果酸含量为3.70 mg/g,平均加标回收率为101.06%,RSD为1.22%;精密度、重复性和稳定性实验的RSD分别为0.02%,0.29%,0.29%.

In order to establish a spectrographic method of measuring oleanolic acid,conditions of UV-spectrophotometry analysis was investigated with vanillin-glacial acetic acid as chromogenic reagent and 545 nm as the determination wavelength.The results showed that the measured concentration value of 8.00 mg/L oleanolic acid standard solution was 8.02 mg/L with 0.3 mL vanillin-glacial acetic acid as chromogenic agent,duration was 15 min under 70℃,linear relation of the oleanolic acid was good in 2.00~16.00 mg/L,and the correlation coefficientwas 0.999 8.Content of oleanolic acid in Akebia trifoliata pericarp was 3.70 mg/g by this analysis method.Standard addition recovery rate was 101.06%,and its relative standard deviation(RSD)was 1.22%.RSD of precision,repeatability and stability was 0.02%,0.29%and 1.29%,respectively.