PDCD4增强鼻咽癌细胞系CNE1对顺铂化疗敏感性机制研究

PDCD4 enhances the sensitivity mechanism of nasopharyngeal carcinoma cell line CNE1 to cisplatin chemotherapy

目的:探讨抑癌基因程序性细胞死亡因子4(the programmed cell death 4,PDCD4)在促进化疗药物顺铂(CDDP)诱导肿瘤细胞凋亡中的作用及可能的机制。方法:CCK8法检测人鼻咽癌系CNE1细胞转染PDCD4前后对CDDP导致的细胞毒性作用影响; Annexin V/FITC和PI凋亡检测转染PDCD4前后对CDDP诱导凋亡的作用; Western blot实验初步探讨其可能的分子机制。结果:PDCD4增强CDDP对人鼻咽癌系CNE1细胞的杀伤作用; PDCD4增加CDDP诱导人鼻咽癌系CNE1细胞凋亡率;机制研究发现PDCD4显著降低人鼻咽癌系CNE1细胞中Twist及MDR1蛋白的表达量,从而促进化疗敏感性。结论:PDCD4通过抑制Twist蛋白增强化疗药物敏感性,提示其参与了鼻咽癌化疗治疗进程。

AIM:To investigate the roles of PDCD4 involved in promoting cisplatin(CDDP)induced tumor cell apoptosis.METHODS:CCK8 method was used to detect the cytotoxicity induced by CDDP in human nasopharyngeal carcinoma cell line(CNE1)before and after PDCD4 transfection.The rate of apoptosis for CDDP-induced nasopharyngeal carcinoma cells was investigated using flow cytometry before and after transfection of PDCD4.Western blot method was used to probe the molecular mechanisms involved in the chemosensitivity.RESULTS:PDCD4 enhanced the killing effect of CDDP on human nasopharyngeal carcinoma cell line CNE1;PDCD4 increased CDDP-induced apoptotic rate of human nasopharyngeal carcinoma cell line CNE1;mechanism study found that PDCD4 significantly decreased the expression of Twist and MDR1 protein,thereby promoting the cell chemosensitivity.CONCLUSION:PDCD4 enhances the sensitivity of chemotherapy drugs by inhibiting Twist protein,suggesting that PDCD4 might serve as a chemotherapy target in nasopharyngeal carcinoma treatment.