ABT-263对顺铂耐药食管癌细胞的抑制作用

Inhibitory effect of ABT-263 on cisplatin-resistant esophageal cancer cells

目的研究ABT-263对顺铂耐药食管癌细胞EC109/CDDP的作用。方法采用MTT法检测ABT-263对耐药细胞EC109/CDDDP及其亲代细胞EC109的细胞毒作用;通过克隆存活实验检测ABT-263对食管癌细胞再生能力的影响;流式细胞术检测细胞凋亡的情况,并通过Western blot检测细胞PARP蛋白的变化。结果 MTT实验结果显示ABT-263对EC109及EC109/CDDP的细胞活力均有显著抑制作用;克隆存活实验结果显示ABT-263可以抑制食管癌细胞的再生能力(P <0.01);流式凋亡结果分析显示ABT-263诱导食管癌细胞发生细胞凋亡(P <0.01);Western blot结果显示ABT-263显著上调细胞PARP蛋白的剪切(P <0.05或P <0.01)。结论ABT-263抑制食管癌EC109/CDDP细胞及其亲代细胞EC109的细胞活力和增殖能力,诱导细胞发生凋亡。

Objective To investigate the effect of ABT-263 on cisplatin-resistant esophageal cancer cell line of EC109/CDDP.Methods The cytotoxicity of ABT-263 in the cisplatin-resistant cell line EC109/CDDP and its parental cell line EC109 was measured by MTT assay.Colony formation assay was used to determine the effect of ABT-263 on cell renewable capacity of esophageal cancer cells.Flow cytometry was adopted to detect cell apoptosis.Western blot was used to investigate the changes of protein expression of PARP.Results MTT assay results showed that ABT-263 significantly inhibited the cell viability of EC109 and EC109/CDDP.Colony formation assay indicated that ABT-263 inhibited the cell renewable capacity of esophageal cancer cells(P﹤0.01).Flow cytometry analysis revealed that ABT-263 induced apoptosis of esophageal cancer cells(P﹤0.01).Western blot results showed that ABT-263 significantly increased the protein expression of cleavage PARP(P<0.05 or P﹤0.01).Conclusion ABT-263 exhibits cytostatic and cytotoxic effects on cisplatin-resistant esophageal cancer cell line EC109/CDDP and its parental cell line EC109,while it induces apoptosis.