磨损颗粒诱导骨溶解的两种动物模型形态学比较研究

Comparative Study on Morphology of Two Animal Models of Wear Particle Induced Osteolysis

目的建立两种磨损颗粒诱导骨溶解模型,比较炎症水平、骨溶解情况以及破骨细胞计数等指标,为骨溶解相关实验动物模型的选择提供依据。方法分别采用小鼠颅骨模型、小鼠气囊植骨模型制备两组磨损颗粒诱导骨溶解模型。通过HE染色法进行形态学观察;利用扫描电子显微镜观察骨溶解情况;采用TRAP染色法进行破骨细胞计数。结果小鼠气囊植骨模型组骨吸收面积为(385.30±5.11)nm2,明显高于小鼠颅骨模型组的(288.60±4.89)nm2(P<0.05);小鼠气囊植骨模型组破骨细胞数目为(16.75±0.53)个,明显高于小鼠颅骨模型组的(9.55±0.48)个(P<0.05)。结论两种动物模型均构建成功;小鼠气囊植骨模型在炎症水平、骨溶解情况及破骨细胞计数等方面均优于小鼠颅骨模型,因此在模拟磨损颗粒诱导的骨溶解病理过程中小鼠气囊植骨模型模拟度更高。

Objective To establish two models of osteolysis induced by wear particles,and to compare the indexes of inflammation,osteolysis and osteoclast count and provide a basis for the selection of animal models of osteolysis.Methods The air pouch mouse model and murine calvarial osteolysis model were established.The morphology was observed by HE staining,the osteolysis was observed by scanning electron microscope,and the osteoclast count was performed by TRAP staining.Results The bone resorption area(385.30±5.11)nm^2 of the air pouch mouse model was significantly higher than that of the murine calvarial osteolysis model(288.60±4.89)nm^2(P<0.05),and the number of osteoclast cells(16.75±0.53)in the air pouch mouse model was significantly higher than that of the murine calvarial osteolysis model(9.55±0.48)(P<0.05).Conclusion All of the two animal models were successfully constructed,and the air pouch mouse model is better than that of the murine calvarial osteolysis model in the level of inflammation,osteolysis and osteoclast count,so the air pouch mouse model is better in the simulation of osteolysis induced by wear particles.