摘要
运用DNA条形码技术,对广东潮州茶园蜘蛛物种进行了分子鉴定。本研究利用基因测序技术获取了凤凰山区域茶园50种蜘蛛的74条线粒体细胞色素C氧化酶亚基I (mitochondrial cytochrome C oxidase subunitⅠ, COⅠ)基因序列。使用邻接法(neighbor joining, NJ)构建系统发育树,运用ABGD(automatic barcode gap discovery)软件对蜘蛛样本进行聚类分析。结果表明:邻接法构建的系统发育树聚类结果与ABGD软件划分结果以及形态分类鉴定结果相一致,运用DNA条形码可以有效地对蜘蛛物种进行分子鉴定。这对茶园蜘蛛疑难物种和新物种的鉴定具有重要意义,在茶园蜘蛛物种多样性的研究中也具有重要价值。由此表明,基于COⅠ基因的DNA条形码对于本研究中所涉及的蜘蛛物种的划分具有较好的区分结果,可以作为一种有效的工具在茶园蜘蛛物种鉴定中进行应用。
Molecular identification of spider species in tea plantation was conducted by using DNA barcoding technology in Chaozhou, Guangdong Province. In this study, 74 COⅠ(mitochondrial cytochrome C oxidase subunit Ⅰ) sequences were amplified from 50 spider species in the tea garden of the Fenghuang Mountains by using gene sequencing technology. The results determined by NJ tree, ABGD(automatic barcode gap discovery) and morphological classification were consistent. Molecular identification of spiders by using the DNA barcodes was effective, which is of great significance for identification of indistinguishable species and new species of tea spiders. At the same time, it is of value in the study of spider species diversity in tea plantations. The results showed that the DNA barcode based on COⅠ gene could be used as an effective tool for identifying spider species in tea plantation.
作者
邢树文
孙延杰
朱慧
查广才
XING Shuwen;SUN Yanjie;ZHU Hui;ZHA Guangcai(College of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou 521041, China)
出处
《植物保护》
CAS
CSCD
北大核心
2019年第1期44-50,80,共8页
Plant Protection
基金
广东省教育厅特色创新类项目(2014KTSCX160)
广东省特色重点学科(生物学)建设项目
潮州市科技计划项目(2014N09)
关键词
蜘蛛
分子鉴定
系统发育树
ABGD
spider
molecular identification
phylogenetic tree
ABGD
