纳流液相分离与电感耦合等离子体质谱联用技术研究新进展

Recent advances in hyphenation of nanoflow liquid phase separation with inductively coupled plasma mass spectrometry

高效微纳液相分离技术如纳流液相色谱、毛细管电泳、微芯片色谱/电泳等与电感耦合等离子体质谱(ICP-MS)检测技术联用,既具有前端分离技术高选择性、高灵敏度、快速、低样品消耗的特点,又结合了后端ICP-MS检测分辨率高、动态范围宽、可绝对定量等优势,正在发展成为一种重要的高内涵联用分析手段。该文对近年来纳流液相分离与ICP-MS联用装置的发展作一系统介绍,对其在化学与生物化学分析等领域的应用予以综述,并展望了该联用技术的发展前景。

High-performance nanoflow liquid-phase separation techniques such as nanoflow liquid chromatography (nanoLC), capillary electrophoresis (CE), and microchip chromatography and electrophoresis (chip-ICP-MS) have been hyphenated with inductively coupled plasma mass spectrometry (ICP-MS) extensively. This hyphenation combines the excellent characteristics of the front-end separation technology, such as high selectivity, sensitivity, and speed, and low sample consumption, and the advantages of back-end ICP-MS, such as high resolution, wide dynamic range, and absolute quantification capability. Thus, such hyphenation is becoming an increasingly important analytical tool. Herein we systematically introduce the recent development of hyphenated nanoLC, CE, and chip-ICP-MS, review its application in chemical and biochemical analysis, and discuss potential future developments of hyphenating these technologies .