白细胞介素16对动脉粥样硬化的影响及其机制研究

Study on effects and mechanism of interleukin 16 on pathogenesis of atherosclerosis

目的分析白细胞介素16(IL-16)在动脉粥样硬化(AS)中的表达,初步探讨其在AS发病机制中的作用。方法收集2015年8月至2016年8月在济宁医学院附属医院就诊并行冠状动脉造影检查确定为AS患者30例作为病例组,另选取同期该院29例体检健康者作为健康对照组。酶联免疫吸附试验(ELISA)检测外周血IL-16水平;逆转录聚合酶链式反应检测两组外周血单个核细胞IL-16的mRNA水平;免疫组化分析AS患者和ApoE-/-小鼠主动脉斑块组织IL-16表达情况;AS小鼠腹腔注射重组IL-16后检测主动脉斑块变化。结果病例组外周血IL-16和IL-16mRNA水平高于健康对照组,差异有统计学意义(P<0.05),人AS斑块中IL-16mRNA高表达。小鼠AS斑块表达IL-16,AS小鼠腹腔注射IL-16后主动脉斑块面积缩小且稳定性增加。结论 IL-16在AS患者外周血和AS小鼠斑块中均高表达,IL-16可能对AS具有保护作用。

:ObjectiveTo analyze the expression of interleukin 16(IL-16)in atherosclerosis(AS)patients,and to study the roles of IL-16 in the pathogenesis of AS.MethodsThirty AS patients in Affiliated Hospital of Jining Medical College from August 2015 to August 2016 were randomly selected as the case group and twenty-nine healthy subjects were selected as the healthy control group.Peripheral blood of the subjects were collected.IL-16 levels were determined with enzyme-linked immunosorbent assay(ELISA).Reverse transcriptase-polymerase chain reaction was applied to analyze IL-16 mRNA level.IL-16 expression in the atherosclerotic plaque samples was detected with immunohistochemical analysis.IL-16 expression in aortic atherosclerotic plaque of AS patients and atherosclerotic ApoE^-/-mice were analyzed by immunohistochemical staining.The aortic plaque changes of AS mice injected intraperitoneally with recombinant IL-16 were detected.ResultsBoth IL-16 protein levels and IL-16 mRNA levels were higher in case group than those of healthy control group,the difference was statistically significant(P<0.05).The IL-16 mRNA was highly expressed in the atherosclerotic plaque.The aortic plaque area of the mice underwent IL-16 intraperitoneal injection were decreased while the plaque stability increased.ConclusionIL-16 levels elevated in both AS patients and AS mice,which suggested that IL-16 might play a protective role against AS.