鸭LXRα基因过表达对原代肝细胞及PC3细胞脂质代谢的效应研究

Effect of Duck LXRαGene Overexpression on Lipid Metabolism in Primary Hepatocytes and PC3 Cells

旨在探究鸭LXRα基因对脂肪代谢的表达调控机制,本试验以pEGFP-N3为载体,构建携带HIS标签的LXRα基因真核过表达载体,并将过表达载体分别转染鸭原代肝细胞及PC3细胞,使用鸭HIS标签试剂盒测定LXRα基因在肝细胞及PC3细胞中的过表达量,分析该基因分别在两种细胞中过表达与各脂质指标含量的相关性,进而探究其对鸭肝细胞及PC3细胞脂质代谢的调控作用。试验结果显示,LXRα基因在两种细胞中过表达与三酰甘油含量(triglyceride,TG)及高密度脂蛋白(high density lipoprotein,HDL)含量均呈极显著正相关关系(P<0.01),与总胆固醇(total cholesterol,TC)含量及低密度脂蛋白含量(low density lipoprotein,LDL)则均呈极显著负相关关系(P<0.01)。本研究揭示了LXRα基因过表达对TG和HDL的合成代谢有重要的正向调控作用,对TC和LDL具有反向调控作用,且在这两种类型的细胞中调控机制相似。这一研究结果将对今后深入研究LXRα基因在脂质代谢网络通路中的作用及解决鸭体脂肪过度沉积等问题奠定理论基础。

To explore the regulation mechanism of duck LXRαgene on fat deposition,in this experiment,the pEGFP-N3 was used as the vector to construct the LXRαgene eukaryotic over-expression vector carried HIS-tag that was transfected into the duck primary hepatocytes and PC3 cells,respectively.The over expression level of LXRαgene in duck hepatocytes and PC3 cells were detected by the duck HIS-tag kit,and the correlation between the over expression of LXRαgene in the two kinds of cells and the lipid index contents was analyzed,and the regulation mechanism of LXRαgene on lipid metabolism in duck hepatocytes and PC3 cells was explored.The results showed that the over expression of LXRαgene in both cells were significantly positively correlated with the contents of triglyceride(TG)and high-density lipoprotein(HDL)(P<0.01),and significantly negatively correlated with the contents of total cholesterol(TC)and low density lipoprotein(LDL)(P<0.01).The results reveal that over expression of LXRαgene has a positive regulatory effect on the anabolism of TG and HDL,and has a negative regulatory effect on TC and LDL,and the regulatory mechanisms in the two types of cells are similar.The results of this study will lay a theoretical foundation for the further study of the role of LXRαgene in the lipid metabolism network pathway and the solve of excessive fat deposition in duck body.