甘肃酒泉甜叶菊黄酮提取工艺优化与含量测定

Study on the Extraction and Content Determination of Stevia Flavonoids from Jiuquan Area of Gansu Province

探究甘肃酒泉产甜叶菊中总黄酮提取的最优工艺,并测定其总黄酮含量。采用正交实验利用高温乙醇超声水浴法提取,利用分光光度法测定其总黄酮含量,芦丁做对照。最佳提取工艺条件为:用80%乙醇,提取功率450W,料液比1∶20,提取时间150min,温度85℃,提取3次,黄酮提取率最高可达到0.88%。用分光光度法测定得到甘肃酒泉产甜叶菊的叶片中总黄酮的含量为0.686mg/g·DW,茎秆中总黄酮的含量为0.822mg/g·DW,高于全国其他地方产甜叶菊,有极大的开发应用价值。

The optimum extraction process of flavonoids from stevia from jiuquan,gansu province was studied.Orthogonal experiment was used to extract the total flavonoids from stevia.The total flavone content was determined by spectrophotometry and rutin was used as control.The results show that through the orthogonal experiment found that high temperature ethanol ultrasonic water bath the best extraction technology conditions for:using 80%ethanol,extract power 450W,material liquid ratio 1:20,150min extraction time,temperature 85℃,extracting 3 times,flavonoids extraction yield can reach 0.88%.The content of flavonoids in leaves of gansu jiuquan stevia was determined by spectrophotometry with 0.686mg/g·DW and that in stalks was 0.822mg/g·DW.