黔产苗药对坐叶药材HPLC指纹图谱研究

Study on HPLC Fingerprint of Miao Medicine Hedyotis uncinella from Guizhou

目的:建立黔产苗药对坐叶药材的高效液相色谱(HPLC)指纹图谱。方法:采用HPLC法。色谱柱为Agilent Eclipse XDB C_(18),流动相为乙腈-0.2%磷酸溶液(梯度洗脱),流速为1.0 mL/min,检测波长为235 nm,柱温为25℃,进样量为15μL。以芦丁为参照,测定10批贵州不同地区对坐叶药材的HPLC图谱,采用《中药色谱指纹图谱相似度评价系统》(2004A版)进行共有峰指认和相似度评价。结果:10批对坐叶药材的HPLC指纹图谱有12个共有峰,相似度均大于0.90。经验证,10批样品HPLC图谱与对照指纹图谱具有较好的一致性。结论:本研究所建HPLC指纹图谱可为对坐叶药材的鉴别和质量评价提供参考。

OBJECTIVE:To establish HPLC fingerprint of Miao medicine Hedyotis uncinella from Guizhou.METHODS:HPLC method was adopted.The determination was performed on Agilent Eclipse XDB C18 column with mobile phase consisted of acetonitrile-0.2%phosphoric acid(gradient elution)at the flow rate of 1.0 mL/min.The detection wavelength was set at 235 nm,and column temperature was 25℃.The sample size was 15μL.Using rutin as reference,HPLC chromatograms of 10 batches of H.uncinella from different areas of Guizhou province were determined.Similarity Evaluation System for TCM Chromatographic Fingerprints(2004A edition)was used to identify common peaks and evaluate similarity.RESULTS:There were 12 common peaks in HPLC fingerprints of 10 batches of H.uncinella,and the similarity was higher than 0.90.After validation,HPLC chromatograms of 10 batches of sample were in agreement with control fingerprints.CONCLUSIONS:Established HPLC fingerprints can provide reference for the identification and quality evaluation of H.uncinella.