宁夏回、汉族人群乙醇代谢关键酶ALDH2基因多态性

Gene polymorphism of alcohol metabolism key enzyme ALDH2 in Ningxia Hui and Han ethnic

目的探讨乙醇代谢关键酶乙醛脱氢酶2(ALDH2)基因G1510A位点单核苷酸多态性在宁夏汉族、回族人群中的分布特征。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术对162名回族和236名汉族人群乙醇代谢关键酶ALDH2基因G1510A(rs671位点)多态性位点进行检测。结果宁夏回族群体乙醇代谢关键酶ALDH2基因G1510A位点各基因型频率及等位基因频率分别为GG:69. 14%,GA:27. 16%,AA:3. 70%,G:82. 72%,A:17. 28%。宁夏汉族群体乙醇代谢关键酶ALDH2基因G1510A位点各基因型频率及等位基因频率分别为GG:71. 19%,GA:25. 00%,AA:3. 81%,G:83. 69%,A:16. 31%。按性别分组,乙醇代谢关键酶ALDH2基因G1510A位点的基因型频率及等位基因频率亦无差异。结论乙醇代谢关键酶ALDH2基因G1510A位点在宁夏回、汉族人群中的分布无明显差异。

Objective To study the frequencies of allele and genotype distribution of key enzyme of alcohol metabolism Gene ALDH2 G1510A polymorphisms in the Hui and Han people in Ningxia.Methods ALDH2 G1510A polymorphisms was examined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 162 hui and 236 han healthy adults.Results ALDH2 G1510A genotypes and alleles frequencies were GG:69.14%,GA:27.16%,AA:3.70%,G:82.72%,A:17.28% of Ningxia Hui ethnic,respectively.ALDH2 G1510A genotypes and alleles frequencies were GG:71.19%,GA:25.00%,AA:3.81%,G:83.69%,A:16.31% of Ningxia Han ethnic.The phenotype frequency and allele gene frequency of ALDH2 G1510A were not significantly different between the female and male people in Ningxia too.Conclusions ALDH2 G1510A distribution in the Hui and Han ethnic from Ningxia is not significantly different.