Yes相关蛋白1和胶原三股螺旋重复蛋白1在三阴性乳腺癌中的表达及其与E-钙黏蛋白表达的相关性

Expressions of Yes-associated protein 1 and collagen triple helix repeat containing 1 in triple-negative breast cancer and their correlations with expression of E-cadherin

目的探讨Yes相关蛋白1(YAP1)和胶原三股螺旋重复蛋白1(CTHRC1)在三阴性乳腺癌(TNBC)中表达的临床意义及与E-钙黏蛋白表达的相关性。方法采用免疫组织化学SABC法检测2006年1月至2017年12月丹东市第一医院73例TNBC患者肿瘤组织和相应癌旁组织中YAP1、CTHRC1的表达,采用χ^2检验和Spearman法分别分析其与患者临床病理特征的关系及其与E-钙黏蛋白表达的相关性。结果YAP1和CTHRC1在TNBC中的表达率分别为71.23%(52/73)、79.45%(58/73),在癌旁组织中的表达率分别为13.70%(10/73)、27.40%(20/73),二者在两组间差异均有统计学意义(χ^2值分别为49.452、39.748,均P<0.01)。YAP1、CTHRC1的表达均与肿瘤分级、临床分期及淋巴结转移相关(YAP1:χ^2值分别为10.244、8.754、6.914,均P<0.05;CTHRC1:χ^2值分别为12.582、13.172、6.400,均P<0.05),与患者年龄、肿瘤直径及绝经状况无关(均P>0.05)。TNBC中YAP1、CTHRC1和E-钙黏蛋白表达均呈负相关(r=-0.371,P=0.001;r=-0.323,P=0.005)。结论YAP1、CTHRC1与TNBC的发生、发展密切相关,可能是通过上皮-间质转化过程参与了TNBC的侵袭。

ObjectiveTo investigate the clinical significances of the expressions of Yes-associated protein 1(YAP1)and collagen triple helix repeat containing 1(CTHRC1)in triple-negative breast cancer(TNBC)and their correlations with expression of E-cadherin.MethodsImmunohistochemical analysis(SABC)was performed to detect expressions of YAP1 and CTHRC1 in 73 specimens of TNBC and adjacent cancer tissues collected from patients in Dandong First Hospital from January 2006 to December 2017.The correlations between the expressions of YAP1 and CTHRC1 and clinicopathologic features and E-cadherin expression were analyzed.ResultsThe expression rates of YAP1 and CTHRC1 in TNBC were 71.23%(52/73)and 79.45%(58/73),and 13.70%(10/73)and 27.40%(20/73)in adjacent cancer tissues,respectively,and the differences were statistically significant(χ^2 values were 49.452 and 39.748,both P<0.01).The expressions of YAP1 and CTHRC1 were related to tumor grade,clinical stage and lymphatic metastasis(YAP1:χ^2 values were 10.244,8.754,and 6.914,all P<0.05;CTHRC1:χ^2 values were 12.582,13.172,and 6.400,all P<0.05),but they were not related to patient's age,tumor diameter and menopausal status(all P>0.05).The expressions of YAP1 and CTHRC1 were negatively correlated with expression of E-cadherin in TNBC(r=-0.371,P=0.001;r=-0.323,P=0.005).ConclusionYAP1 and CTHRC1 is closely related to the occurrence and development of TNBC and may participate in the invasion of TNBC through epithelial mesenchymal transition.