海参酶解液对斑马鱼糖尿病并发抑郁样行为的改善作用

Effect of Sea Cucumber Hydrolysis Extract on Depression-like Behavior of Zebrafish Induced by Diabete

【目的】探究海参酶解液对高糖高脂所致的斑马鱼抑郁样行为的改善作用。【方法】将斑马鱼随机分为对照组、模型组各1组,和海参酶解液高剂量(1.0m L/L)治疗组、低剂量(0.5m L/L)治疗组各1组。每天利用高浓度葡萄糖(质量分数2%)系统浸泡及高胆固醇(质量分数10%)饲料饲喂诱导斑马鱼高糖高脂模型,高低浓度海参酶解液浸泡治疗。14 d后进行新鱼缸潜水行为实验,取鱼体匀浆进行组织各生化指标的检测,包括葡萄糖(Glucose, Glu)、总胆固醇(Total cholesterol, TC)、皮质醇(Cortisol, Cor)浓度。【结果】新鱼缸行为实验中,与对照组相比较,模型组斑马鱼的行为学实验都有明显差异,其中焦虑型行为潜伏期、冻结次数和冻结时间等抑郁型行为增加,探究型行为顶部转移和顶部时间减少,而1.0 mL/L高剂量海参酶解液可以逆转这些变化。模型组葡萄糖、总胆固醇、皮质醇浓度相比对照组显著升高,而给予海参酶解液治疗后,高剂量治疗组对模型组的葡萄糖、总胆固醇及皮质醇浓度均有显著降低的作用。【结论】海参酶解液可以通过改善高糖高脂诱导的糖尿病模型中糖脂水平来改善抑郁样行为和应激激素。

【Objective】To explore the effect of sea cucumber enzymatic hydrolysate(SCEH)on the depression-like behavior in the diabetic model of zebrafish induced by high sugar and high fat.【Methods】Zebrafish were randomly divided into the control group,model group,model plus sea cucumber high(1.0 mL/L)and low dose(0.5 mL/L)treatment groups.The fish was systemically soaked in high concentration glucose(2%),and was fed by high cholesterol(10%)to induce a diabetic mode.Then,high and low doses of SCEH were used for treatment by soaking.After 14 days,behavioral tests in Novel Tank Test(NTT)were carried out.The fish body was used to detect the biochemical indicators,including concentrations of glucose(Glu),total cholesterol(TC),and cortisol(Cor).【Results】In NTT,the behavioral responses from zebrafish in the model group were significantly different from those in the control group.The depression-like behavior in NTT,such as anxiety-like behavior latency,freezing bouts and freezing time were increased,while exploring behavior top transitions and time in top were decreased in the model group.These changes were attenuated by higher dose of SCEH.The concentrations of glucose,total cholesterol and cortisol were significantly increased in the model group,which were significantly reduced by treatment with SCEH at high-dose when compared to the model group.【Conclusion】SCEH may improve the depression-like behavior and stress hormone by the improvement of related lipid and sugar parameters in the diabetic model induced by high sugar and high fat.