摘要
Objective:To investigate the effect of Dingjifumai Decoction(DJFM)on Electrocardiogram(ECG)and sodium potassium pump in rats with ventricular arrhythmia.Methods:Forty healthy male SD rats(200±20g)were randomly divided into blank group,model group,Metoprolol group and DJFM group.Ten rats in each group were fed with normal diet and free drinking water.Each group was given gavage,and the amount of gavage in each group was calculated according to body weight.In the model group,0.001%Aconitine was injected into the tail vein at 30ug/kg.In the Metoprolol group,Metoprolol suspension was given according to the standard of 5.2mg/kg per day.In the DJFM group,DJFM was given at 17.6g/kg per day.After 2 weeks of administration,the biologic experiment system BL-420F was used to monitor the II lead ECG curve,and the ECG changes were observed and recorded.Then,the left ventricle of the rat was taken,and part of the heart tissue sodium potassium pump was detected.Results:(1)The effect of DJFM on ECG of rats with ventricular arrhythmia:After intravenous injection of aconitine,the incidence of Ventricular Premature beat(VP),Ventricular Tachycardia(VT),Ventricular Fibrillation(VF)in the model group was 100%,suggesting that the model building of rats with ventricular arrhythmia was successful.(2)VP,VT,and VF time:Compared with model group,DJFM group and Metoprolol group can significantly delay the VP,VT and VF,the difference was statistically significant(P<0.05).The effect of DJFM group and Metoprolol group on delaying the appearance of VP,VT and VF was the same,there was no significant difference(P>0.05).(3)The effect of DJFM on sodium potassium pump in rat ventricular arrhythmia heart tissues:Compared with the blank group,the sodium potassium pump value in the model group was significantly decreased,and the difference was statistically significant(P<0.05).Compared with the model group,the sodium potassium pump value of the tissues in the Metoprolol group and the DJFM group increased,and the difference was statistically significant(P<0.05).There was no significant difference in sodium potassium pump between the Metoprolol group and the DJFM group(P>0.05).Conclusion:1.The rat model of ventricular arrhythmia can be successfully prepared by intravenous injection of Aconitine.2.DJFM can prolong the occurrence time of cardiac arrhythmias caused by aconitine in rats,such as VP,VT,VF,et al.The mechanism may be related to fast Na^+channel,and it may prevent and control arrhythmias by inhibiting Na^+influx and reducing the fast response cellular self-discipline.3.DJFM can protect the myocardial tissue sodium potassium pump,which can protect the myocardial cells and improve the myocardial metabolism.
目的:探讨定悸复脉汤对室性心律失常大鼠心电图及Na^+-K^+-ATP酶的影响。方法:将健康雄性SD大鼠40只(200±20g)随机分为空白组、模型组、美托洛尔组及定悸复脉汤组,每组10只,均喂养普通饲料和自由饮水,各组均给予灌胃,各组灌胃量均按体重计算。模型组给予尾静脉注射0.001%乌头碱30ug/kg,美托洛尔组按每天5.2mg/kg的规格灌服美托洛尔悬液,定悸复脉汤组按每天17.6g/kg剂量给予定悸复脉汤灌胃。给药2周后,通过生物机能实验系统BL-420F监测Ⅱ导联心电图曲线观察并且记录心电图的变化,然后取大鼠左心室,部分行心脏组织的Na^+-K^+-ATP酶检测。结果:(1)定悸复脉汤对室性心律失常大鼠心电图的影响:静脉推注乌头碱后,模型组VP、VT、VF发生率为100%,提示室性心律失常大鼠模型造模成功。(2)室性早搏(VP)、室性心动过速(VT)、室性颤动(VF)出现时间:与模型组比较,定悸复脉汤组和美托洛尔组可显著推迟VP、VT、VF出现时间,差异具有统计学意义(P<0.05),定悸复脉汤组与美托洛尔组在推迟VP、VT、VF出现时间方面作用相同,无明显差异(P>0.05)。(3)定悸复脉汤对大鼠室性心律失常心脏组织Na^+-K^+-ATP酶的影响:与空白组比较,模型组组织Na^+-K^+-ATP酶值明显降低,差异具有统计学意义(P<0.05)。与模型组比较,美托洛尔组和定悸复脉汤组的组织Na^+-K^+-ATP酶值升高,差异具有统计学意义(P<0.05);美托洛尔组与定悸复脉汤组组织Na^+-K^+-ATP酶值无明显差异(P>0.05)。结论:1.采用静脉推注乌头碱的方法可以成功制备室性心律失常大鼠模型。2.定悸复脉汤可延长乌头碱所致大鼠VP、VT、VF等心律失常的出现时间,其机制可能与快Na^+通道相关,可能通过抑制Na^+内流而降低快反应细胞自律性而防治心律失常。3.定悸复脉汤能保护心肌组织Na^+-K^+-ATP酶,具有保护心肌细胞,从而改善心肌代谢作用。
