摘要
本研究以桑黄为材料,筛选出一种快速直接提取子实体DNA的方法。利用CTAB法、试剂盒及硅珠DNA纯化技术分别对桑黄子实体基因组DNA进行提取,结果表明,硅珠DNA纯化技术的提取效果最好,试剂盒方法次之。经过PCR扩增和测序得到桑黄的rDNA ITS序列,通过BLAST比对及系统进化树构建,发现所检测的样品为哈尔蒂木层孔菌Phellinus hartigii。
Using Phellinus igniariusas as research materials,a method for rapidly extracting DNA from Phellinus igniarius was screened.The genomic DNA of Phellinus igniarius was respectively extracted by CTAB method,DNA extract kit and silica bead DNA purification technology.The results showed that the extraction method of silica bead DNA purification technology was the best,and the kit method was the second.The ITS gene sequence of Phellinus igniarius was obtained by PCR amplification and sequencing.By the BlAST sequence alignment and the construction of phylogenetic tree,we found the detected sample was Phellinus hartigii.
作者
陈力群
谭晴晴
陈雪燕
刘利平
刘国霞
胡悦
范阳阳
刘艳艳
步迅
张全芳
Chen Liqun;Tan Qingqing;Chen Xueyan;Liu Liping;Liu Guoxia;Hu Yue;Fan Yangyang;Liu Yanyan;Bu Xun;Zhang Quanfang(High School Attached to Shandong Normal University,Jinan 250014,China;Bio-Tech Research Center,Shandong Academy of Agricultural Sciences,Jinan 250100,China;Shandong Academy of Agricultural Sciences Community Health Service Station of Quanfu Officein Licheng District of Jinan City,Jinan 250100,China)
出处
《山东农业科学》
2019年第1期18-21,31,共5页
Shandong Agricultural Sciences
基金
山东省重点研发计划项目"30种柴胡SNP指纹图谱芯片的开发及应用"(2018GSF119002)
山东省创新公共服务平台计划项目"中药分子鉴定公共服务平台"(2018JGX111)
山东省农业科学院农业科技创新工程项目"山东道地药材产业关键技术创新与应用"(CXGC2018D03)
关键词
桑黄
DNA提取
ITS
分子检测
Phellinus igniarius
DNA extraction
ITS
Molecular detection