摘要
为探明菌株TNF53的分类地位及其抑菌活性,采用试剂盒法、滤纸片扩散法和对峙培养法对从云南榧树叶片上分离得到的内生真菌TNF53进行分子鉴定及其抑菌活性研究。初步鉴定,TNF53为炭疽菌(Colletotrichum),该菌粗提物对三七丝核病菌、辣椒炭疽病菌、辣椒镰刀病菌、水稻稻瘟病菌和白菜黑斑病菌均表现出抑菌活性,其中对三七丝核病菌的抑菌活性最明显。以上结果表明该菌对植物病原真菌具有广谱抗性。
To identify the taxonomic status and antibacterial activity of TNF53 strain,the molecular identification and antibacterial activity of endophytic fungus TNF53 isolated from leaves of Yunnan eucalyptus were studied by kit method,filter paper diffusion method and sputum culture method.The TNF53 was initially identified as Colletotrichum.The crude extract of the strain showed antibacterial activity against Verticillium cinnabarium,Colletotrichum capsici,Fusarium solani,Magnaporthe grisea and Alternaria brassicae,especially against Verticillium cinnabarium.The above results indicated that the strain had broad-spectrum resistance to phytopathogenic fungi.
作者
隋国强
马长乐
张金丽
李博文
李靖
Sui Guoqiang;Ma Changle;Zhang Jinli;Li Bowen;Li Jing(Department of Life Sciences,Southwest Forestry University,Kunming 650224,China;College of Landscape Architecture,Southwest Forestry University,Kunming 650224,China)
出处
《山东农业科学》
2019年第1期134-137,共4页
Shandong Agricultural Sciences
基金
国家自然科学基金项目(31360037)
西南林业大学博士科研启动基金项目
关键词
内生真菌
分子鉴定
云南榧树
抑菌活性
Endophytic fungus
Molecular identification
Torreya yunnanensis
Antibacterial activity