东北地区鸡源多重耐药大肠杆菌整合子的检测

Detection of Integrons in Multi-drug Resistant Isolates of Escherichia coli from Chicken in Northeastern China

[目的]了解东北地区鸡源大肠杆菌耐药性的流行特征以及整合子-基因盒系统的耐药机制。[方法]在检测耐药性的基础上,采用PCR方法对整合酶和整合子进行检测和克隆测序,并且对检测结果和耐药基因盒与GenBank数据库的序列进行比对和分析。[结果]413株分离菌中整合子检出率为71.91%,整合酶Ⅰ和整合酶Ⅱ的检出率分别为65.52%和54.48%,整合酶Ⅲ未检出。序列分析得出,共包含6种整合子,各自携带不同组合的基因盒。[结论]Ⅰ型整合子在细菌耐药性的传播过程中发挥着至关重要的作用。

[Objective]To investigate the prevalence characteristics of drug-resistant Escherichia coli isolates in Northeastern China and the drug-resistant mechanism of integron-gene cassettes system.[Method]On the basis of drug-resistant detection,integrase and integron were detected,cloned and sequenced by PCR method.The detection results and drug-resistant gene cassettes were blasted with sequences with GenBank database.[Result]The detection rate of integron among 413 strains of isolates was 71.91%.The detection rates of integrase Ⅰ and integrase Ⅱ were 65.52% and 54.48% respectively.Integrase Ⅲ was not detected.There were six types of integrons by sequencing analysis,which carried different combinations of gene cassettes.[Conclusion]Integron Ⅰ played an important role in the propagation process of bacterial resistance.