恒河猴与食蟹猴血清多重细胞因子检测及分析

Measurement and comparative analysis of multiple serum cytokines in Rhesus and Cynomolgus monkeys

目的测定和比较健康恒河猴与食蟹猴基础血清细胞因子水平。方法分别采集15只健康恒河猴和15只健康食蟹猴血清,采用luminex xMap技术测定血清43种细胞因子的浓度,根据正态性检验(W检验)结果选择随机t检验或非参数检验(Mann-Whitney test)比较群体间差异。结果测定的43种细胞因子中,1种促炎症因子(IL-15)、3种抑炎症因子(IL-1RA、IL-6Rα和s CD27)和1种趋化因子(CCL5/RNATES)存在显著性群体间差异。结论研究结果表明,恒河猴与食蟹猴基础血清细胞因子水平差异不显著,用于生物医学研究中具有一定的可比性。同时,本研究结果可为相关研究提供基础数据。

Objective To detect and compare the clinical outcomes of multiple serum cytokines in healthy Rhesus and Cynomolgus monkeys.Methods Fifteen healthy Rhesus and 15 healthy Cynomolgus monkeys were chosen for sample collection.Serum concentrations of 43 cytokines were measured using xMap technologies.The results were analyzed by t-test or non-parametric Mann-Whitney U-test according to the W-test for normality.Results Among 43 cytokines,one pro-inflammatory cytokine(IL-15),three anti-inflammatory cytokines(IL-1RA,IL-6Rα,and sCD27)and one chemokine(CCL5/RANTES)were significantly different between Rhesus and Cynomolgus monkeys.Conclusions There is no significant difference in basic serum cytokine levels between Rhesus and Cynomolgus monkeys,which is comparable in biomedical research.The results of this study provide basic data for related studies.