激活AMPK对妊娠高血压大鼠HO-1水平、血压和尿蛋白的影响

Effects of AMPK activation on HO-1 level,blood pressure and urinary protein in gestational hypertensive rats

目的探讨激活AMP依赖的蛋白激酶(AMPK)对妊娠高血压大鼠的保护作用。方法建立妊娠高血压大鼠模型,用AMPK特异性激动剂阿卡地新(AICAR)干预后,分为正常妊娠组、亚硝基左旋精氨酸甲酯(L-NAME)组、L-NAME+AICAR组,测量大鼠血压,ELISA测定24h尿蛋白,胎盘组织苏木精-伊红(HE)染色,免疫组织化学检测AMPK、血红素氧合酶-1(HO-1)表达的细胞定位,Western blot检测胎盘组织AMPK、HO-1表达水平。结果与正常妊娠组孕鼠比较,L-NAME组孕鼠血压升高,24h尿蛋白水平显著增加,AMPK、HO-1表达水平降低(P<0.05)。与L-NAME组比较,L-NAME+AICAR组血压降低,24h尿蛋白减少,AMPK、HO-1表达水平升高(P<0.05)。结论激活AMPK可提高妊娠高血压大鼠HO-1水平、降低血压及减少24h尿蛋白。

Objective To investigate the protective role of AMPK activation in gestational hypertensive rat model.Methods Rat model was established through administration of L-nitro-arginine methylester(L-NAME)and intervened by 5-aminoimidazole-4-carboxamide1-β-D-ribofuranoside(AICAR),a AMPK specific activator.There were three groups,the normal group,the L-NAME group and the L-NAME+AICAR group.Blood pressure was monitored,and 24 h urine protein was measured by ELISA.The placental tissue was stained with hematoxylin and eosin(HE)and the expression of AMPK and HO-1 was detected by immunohistochemistry staining and Western blot.Results Comparing with the L-NAME group,L-NAME+AICAR rats showed decreasing blood pressure and reducing 24 h urine protein level with increasing AMPK and HO-1 expression(P<0.05).Conclusion AMPK activation can up-regulate the expression of HO-1,reduce the blood pressure and 24 h urinary protein in gestational hypertensive rats.