TLR4基因rs1927914位点多态性与甲状腺疾病的关联分析

Correlation analysis of the polymorphism of TLR4 gene rs1927914 locus with thyroid diseases

目的探讨Toll样受体4(TLR4)基因的单核苷酸多态性与Graves病(GD)、桥本甲状腺炎(HT)、单纯性甲状腺肿(SG)的相关性。方法选取2016年河北省沧州市居民甲状腺疾病调查的GD患者(GD组,n=94)、HT患者(HT组,n=54)、SG患者(SG组,n=56)及对照组为研究对象,应用PCR酶切鉴定结合直接测序方法进行基因分型。结果等位基因C和T在3种甲状腺疾病和对照组中比较,差异均无统计学意义(P>0.05);3种基因型CC、CT、TT在GD组、HT组中的分布与对照组比较,差异均无统计学意义(P>0.05);SG组与对照组比较,隐性遗传模型显示基因突变与疾病呈正相关(P=0.040,OR=1.923,95%CI:1.025~3.609)。结论 TLR4基因多态性可能与GD、HT的发病风险无相关性,携带TT基因型可能会增加SG的发病风险。

Objective To investigate the correlation between single nucleotide polymorphism(SNP)of Toll-like receptor 4(TLR4)gene and Graves′disease(GD),Hashimoto′s thyroiditis(HT)or simple goiter(SG).Methods Data was collected from GD patients(the GD group,n=94),HT patients(the HT group,n=54),SG patients(the SG group,n=56),and healthy people(the control group)of 2016 Thyroid Disease Survey in Hebei Cangzhou.Genetic typing was detected by polymerase chain reaction-restriction combined with direct sequencing.Results There was no significant difference in allele C and T at TLR4-C/T between the three thyroid diseases and the control group(P >0.05).The distribution of CC,CT,TT genotypes in the GD group and the HT group was not significantly different from the control group(P >0.05).And by comparing the SG group and the control group,the recessive genetic model showed that gene mutation was positively correlated with the disease(P=0.040,OR=1.923,95%CI:1.025-3.609).Conclusion There might be no correlation between TLR4 gene polymorphism and the incidence of GD or HT,and carrying with the TT genotype may increase the incidence of SG.