GSK-3β和E-cadherin在子宫内膜腺癌中的表达和意义

Expression of GSK-3β and E-cadherin in endometrial cancer and its significance

目的研究糖原合成酶激酶-3(GSK-3β)和E-钙粘蛋白(E-cadherin)在子宫内膜腺癌组织中的表达水平,了解其表达水平与生物学特性、预后的关联。方法应用免疫组织化学SP技术检测55例子宫内膜腺癌组织,19例子宫内膜不典型增生组织,12例正常增殖期子宫内膜组织中GSK-3β和E-cadherin的表达。结果在子宫内膜腺癌组织、子宫内膜不典型增生组织和正常增殖期子宫内膜组织中GSK-3β和E-cadherin的阳性表达呈逐渐增高趋势,差异有统计学意义(χ2=15.640,P=0.000)。GSK-3β在子宫内膜腺癌组织中的表达随患者FIGO分期的进展呈下降趋势(P<0.05),而与组织学病理分级、是否浸润肌层、淋巴结转移、年龄无明显相关(P>0.05);E-cadherin在子宫内膜腺癌组织中的表达随患者组织学病理分级的增高呈下降趋势(P<0.05),而与临床分期、淋巴结转移、是否浸润肌层、年龄无明显相关(P>0.05)。GSK-3β和E-cadherin的表达呈正相关(r=0.516,P=0.000)。结论 GSK-3β和E-cadherin可能在子宫内膜腺癌发生、发展中发挥着既独立又协同的抑癌基因作用。

Objective To investigate the expression of GSK-3βand E-cadherin in human endometrial adenocarcinoma(EAC)and analyze their relationship with clinicopathological features.Methods The GSK-3βand E-cadherin protein in normal proliferative endometrium group(12 cases),endometrial atypical hyperplasia group(19 cases),endometrial adenocarcinoma group(55 cases)were detected by immunohistochemical SP technique.Results The positive expression rates of GSK-3βprotein and E-cadherin protein increased gradually in normal proliferative endometrium,endometrial atypical hyperplasia,endometrial adenocarcinoma tissues,and there were statistically significant differences among three groups(χ^2=15.640,P=0.000).The positive expression of GSK-3βdecreased with the increasing clinical stages(P<0.05),and no difference among pathological grading,age,lymph node metastasis and cancerous tissue infiltration depth(P >0.05).The positive expression of E-cadherin decreased with the increasing histological grade(P<0.05),and no difference between the expression of E-cadherin and clinical stages,myometrial invasion,lymph node metastasis and age(P >0.05).GSK-3βand E-cadherin expression in endometrial carcinoma showed a positive correlation(r=0.516,P=0.000).Conclusion GSK-3βand E-cadherin may play an independent and congenerous anti-oncogene role in the incidence and development of endometrial carcinogenesis.