脑梗死患者外周血调节性B细胞水平及其与颈动脉粥样硬化严重程度的相关性

Peripheral blood regulatory B cell levels in patients with cerebral infarction and their correlation with the severity of carotid atherosclerosis

目的探讨脑梗死患者外周血调节性B细胞(Bregs)水平变化及其与颈动脉粥样硬化严重程度的相关性。方法选取2017-09-01—2018-05-01十堰市太和医院收治的动脉粥样硬化性脑梗死患者50例,并选择同期健康体检者50名为对照组。采用流式细胞术(FCM)检测外周血B细胞中CD19+CD5+CD1d+B细胞(Bregs)所占B细胞的比例;采用ELISA法测定Bregs培养液上清中白细胞介素-10(IL-10)及转化生长因子-β1(TGF-β1)水平。分析脑梗死患者外周血Bregs水平变化以及其与颈动脉粥样硬化程度的关系。结果脑梗死患者外周血Bregs水平及其分泌的IL-10、TGF-β1水平较对照组降低(P<0.05);随颈动脉粥样硬化严重程度加重,脑梗死患者外周血Bregs水平及IL-10、TGF-β1水平逐渐降低(均P<0.05)。结论动脉粥样硬化性脑梗死患者外周血Bregs水平低于健康人;Bregs水平与动脉粥样硬化性脑梗死患者颈动脉粥样硬化严重程度密切相关;外周血Bregs水平的降低以及功能的丧失参与了脑梗死的发生和发展,其机制可能与机体免疫平衡被打破有关。

Objective To investigate the level of peripheral blood regulatory B cells(Bregs)in patients with cerebral infarction and its correlation with the severity of carotid atherosclerosis.Methods Fifty patients with atherosclerotic cerebral infarction who were admitted to the Department of Neurology,Taihe Hospital from January 2017 to May 2018 were selected as the study group,and 50 healthy controls were selected as the control group.Flow cytometry(FCM)was used to detect the proportion of CD19^+CD5^+CD1d^+B cells(Bregs)in B cells.Interleukin-10(IL-10)and transforming growth factor-β1(TGF-β1)levels in supernatant of Bregs culture medium were determined by ELISA.Then we compared the data between the 50 healthy people and the 50 patients with cerebral infarction and carotid atherosclerosis.Results The levels of Bregs,IL-10 and TGF-β1 in the patients with cerebral infarction were significantly lower than those in the healthy people(P<0.05).As the severity of carotid atherosclerosis worsened,the levels of peripheral blood Bregs and the levels of cytokines IL-10 and TGF-β1 gradually decreased(P<0.05).Conclusions Decreased levels and loss of function of peripheral blood Bregs are involved in the development and progression of cerebral infarction,which may be related to the disruption of the body's immune balance.